Figures & data
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Table 1 Saturation Solubility Of HupA In Different Oils, Surfactants And Co-Surfactants
Table 2 Factor Level And Response Data For Full-Factorial Study
Figure 2 Pseudo-ternary phase diagrams for NE optimization. (A) Smix 1:2, (B) Smix 1:1, and (C) Smix 2:1.
![Figure 2 Pseudo-ternary phase diagrams for NE optimization. (A) Smix 1:2, (B) Smix 1:1, and (C) Smix 2:1.](/cms/asset/5f2a230c-b48b-41ee-a978-f21535d4bcf6/dijn_a_12191072_f0002_b.jpg)
Figure 3 Response surface plots showing significant interaction effects. Globule size (A–C) and PDI (D–F) as the effect of formulation variables.
![Figure 3 Response surface plots showing significant interaction effects. Globule size (A–C) and PDI (D–F) as the effect of formulation variables.](/cms/asset/6cecb705-601a-46c2-aba3-f022563ba667/dijn_a_12191072_f0003_c.jpg)
Figure 5 Characterization parameters of optimized NEs (A–C) Droplet size distribution of NE, HupA-NE and Lf- HupA-NE, (D–F) zeta potential of NE, HupA-NE and Lf- HupA-NE.
![Figure 5 Characterization parameters of optimized NEs (A–C) Droplet size distribution of NE, HupA-NE and Lf- HupA-NE, (D–F) zeta potential of NE, HupA-NE and Lf- HupA-NE.](/cms/asset/a2a19e8d-635e-4d3b-a000-c830d0bcc8b2/dijn_a_12191072_f0005_c.jpg)
Figure 8 Protein levels of transporters in confluent hCMEC/D3 cells. Lane 1, PBS; Lane 2, HupA-NE; Lane 3, Lf-HupA-NE; and Lane 4, HupA solution.
![Figure 8 Protein levels of transporters in confluent hCMEC/D3 cells. Lane 1, PBS; Lane 2, HupA-NE; Lane 3, Lf-HupA-NE; and Lane 4, HupA solution.](/cms/asset/b961e622-b91a-42b0-9829-b11209d0e7a5/dijn_a_12191072_f0008_c.jpg)
Figure 9 Effects of (A) transporter inhibitors and (B) endocytosis inhibitors on cellular uptake of HupA-NE and Lf-HupA-NE. Values represent the mean±SD (n=3). Statistically significant in comparison with normal control **p<0.01, ***p<0.001.
![Figure 9 Effects of (A) transporter inhibitors and (B) endocytosis inhibitors on cellular uptake of HupA-NE and Lf-HupA-NE. Values represent the mean±SD (n=3). Statistically significant in comparison with normal control **p<0.01, ***p<0.001.](/cms/asset/1c8e62d3-24c8-497d-bc4f-b392f5ca3ac3/dijn_a_12191072_f0009_c.jpg)
Figure 10 Nasal mucosa of rats treated with (A) normal saline (B) 1% deoxycholic acid sodium solution (C, E, G) HupA-NE on days 1, 7, 14 (D, F, H) Lf-HupA-NE on days 1, 7.14.
![Figure 10 Nasal mucosa of rats treated with (A) normal saline (B) 1% deoxycholic acid sodium solution (C, E, G) HupA-NE on days 1, 7, 14 (D, F, H) Lf-HupA-NE on days 1, 7.14.](/cms/asset/5ca8ad7b-9598-4c1d-9ba3-c724ce264792/dijn_a_12191072_f0010_c.jpg)
Figure 11 Fluorescence in the brain (A–C) No treatment, (D–F) free RhB-HupA, (G–I) RhB-HupA-NE and (J–L) Lf-RhB-HupA-NE. Red: RhB- HupA, Blue: cell nucleus.
![Figure 11 Fluorescence in the brain (A–C) No treatment, (D–F) free RhB-HupA, (G–I) RhB-HupA-NE and (J–L) Lf-RhB-HupA-NE. Red: RhB- HupA, Blue: cell nucleus.](/cms/asset/2d252e29-25cb-44b5-9d5c-40fe1d9498e7/dijn_a_12191072_f0011_c.jpg)
Table 3 Pharmacokinetic Parameters Of HupA-NE, Lf- HupA-NE And HupA Solution In The Brain
Table 4 Pharmacokinetic Parameters Of HupA-NE, Lf- HupA-NE And HupA Solution In The Plasma