Figures & data
Table 1 Different batches of SPs prepared with HPMCP, Eudragit L 100 and Eudragit S 100 polymer
Table 2 Effect of formulation variables on yield, encapsulation efficiency and particle size of HPMCP, Eudragit L 100 and Eudragit S 100 SPs prepared by double emulsion solvent evaporation technique
Figure 1 Effect of pH 1.2 on the aggregation of optimized papain loaded enteric SPs of HPMCP (A), Eudragit L 100 (B) and Eudragit S 100 (C).
Figure 2 Effect of pH on the release of enzyme from the optimized formulation of papain loaded SPs of HPMCP (HS4), Eudragit L 100 (LS4) and Eudragit S 100 (SS4) at pH 6, 6.8 and 7.4 respectively.
Figure 3 SDS-PEGE analysis of papain samples. Lane 1: molecular weight markers (14–94kDa); lane 2: papain (reference standard); lane 3, 4 and 5: papain released from optimized formulations of papain loaded HPMCP, Eudragit L 100 and Eudragit S 100 SPs respectively.
Figure 4 Intrinsic fluorescence spectra of papain (reference standard) and papain released from optimized formulations of papain loaded HPMCP, Eudragit L 100 and Eudragit S 100 SPs respectively.
Figure 5 The FTIR spectra Eudragit L 100, Eudragit S 100, HPMCP, papain powder and optimized formulation of papain loaded HPMCP, Eudragit L 100 and Eudragit S 100 SPs.
Figure 6 XRD pattern of Eudragit S 100, Eudragit L 100, HPMCP, papain powder and optimized formulation of papain loaded HPMCP, Eudragit L 100 and Eudragit S 100 SPs.
Figure 7 The DSC thermograms of Eudragit S 100, Eudragit L 100, HPMCP, papain powder and optimized formulations of papain loaded Eudragit S 100, Eudragit L 100 and HPMCP SPs.
Figure 8 SEM micrographs of optimised formulations of papain loaded HPMCP (A), Eudragit L 100 (B) and Eudragit S 100 SPs (C).
Table 3 Stability of free papain and optimised formulations of papain (HS4, LS4 and SS4) under accelerated storage conditions (40°C ± 2°C/75% ± 5% RH)
Table 4 Stability of free papain and optimised formulations of papain (HS4, LS4 and SS4) under room temperature storage (30°C ± 2°C/65% ± 5% RH)