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Original Research

Electrospun PGA/gelatin nanofibrous scaffolds and their potential application in vascular tissue engineering

Hadi Hajiali1 Nanomedicine and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IranCorrespondence[email protected]

,

Shapour Shahgasempour1 Nanomedicine and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

,

M Reza Naimi-Jamal2 Department of Chemistry, Iran University of Science and Technology, Tehran, Iran

&

Habibullah Peirovi1 Nanomedicine and Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Figures & data

Figure 1 Scanning electron microscopic morphology of electrospun PGA/gelatin nanofibers (2000× and 20,000× magnification). (A) Pure PGA, (B) PGA/10 wt% gelatin, (C) PGA/30 wt% gelatin, and (D) PGA/50 wt% gelatin. (E) Mean diameter (± standard deviation) variations of electrospun PGA/gelatin fibers, (F) mean area (± standard deviation) between fibers, and (G) distribution of fiber orientation angles (n = 50).

Notes: ***P < 0.001, significantly different from the previous group.

Abbreviation: PGA, polyglycolic acid.

Figure 1 Scanning electron microscopic morphology of electrospun PGA/gelatin nanofibers (2000× and 20,000× magnification). (A) Pure PGA, (B) PGA/10 wt% gelatin, (C) PGA/30 wt% gelatin, and (D) PGA/50 wt% gelatin. (E) Mean diameter (± standard deviation) variations of electrospun PGA/gelatin fibers, (F) mean area (± standard deviation) between fibers, and (G) distribution of fiber orientation angles (n = 50).Notes: ***P < 0.001, significantly different from the previous group.Abbreviation: PGA, polyglycolic acid.

Figure 2 (A) Attenuated total reflectance-Fourier transform infrared spectra of PGA/gelatin scaffolds and (B) differential scanning calorimetric curves of PGA/gelatin scaffolds.

Abbreviation: PGA, polyglycolic acid.

Figure 2 (A) Attenuated total reflectance-Fourier transform infrared spectra of PGA/gelatin scaffolds and (B) differential scanning calorimetric curves of PGA/gelatin scaffolds.Abbreviation: PGA, polyglycolic acid.

Figure 3 (A) Young’s modulus of PGA/gelatin scaffolds, mean ± standard deviation (n = 3), (B) tensile strength of PGA/gelatin scaffolds, mean ± standard deviation (n = 3), and (C) elongation at break of PGA/gelatin scaffolds, mean ± standard deviation (n = 3).

Notes: *P < 0.05; ***P < 0.001, significantly different compared with the previous group.

Abbreviation: PGA, polyglycolic acid.

Figure 3 (A) Young’s modulus of PGA/gelatin scaffolds, mean ± standard deviation (n = 3), (B) tensile strength of PGA/gelatin scaffolds, mean ± standard deviation (n = 3), and (C) elongation at break of PGA/gelatin scaffolds, mean ± standard deviation (n = 3).Notes: *P < 0.05; ***P < 0.001, significantly different compared with the previous group.Abbreviation: PGA, polyglycolic acid.

Figure 4 Cell viability (MTT results) on PGA/gelatin scaffolds after days 1, 3, and 5. Mean ± standard deviation (n = 3).

Notes: *P < 0.05; **P < 0.01.

Abbreviation: PGA, polyglycolic acid.

Figure 4 Cell viability (MTT results) on PGA/gelatin scaffolds after days 1, 3, and 5. Mean ± standard deviation (n = 3).Notes: *P < 0.05; **P < 0.01.Abbreviation: PGA, polyglycolic acid.

Figure 5 Scanning electron micrographs of human umbilical vein endothelial cells cultured on PGA/gelatin scaffolds (2000× magnification). (A) Pure PGA, (B) PGA/10 wt% gelatin, (C) PGA/30 wt% gelatin, and (D) PGA/50 wt% gelatin.

Figure 6 Scanning electron micrographs of human umbilical artery smooth muscle cells cultured on PGA/gelatin scaffolds (2000× magnification). (A) Pure PGA, (B) PGA/10 wt% gelatin, (C) PGA/30 wt% gelatin, and (D) PGA/50 wt% gelatin.

Abbreviation: PGA, polyglycolic acid.

Figure 6 Scanning electron micrographs of human umbilical artery smooth muscle cells cultured on PGA/gelatin scaffolds (2000× magnification). (A) Pure PGA, (B) PGA/10 wt% gelatin, (C) PGA/30 wt% gelatin, and (D) PGA/50 wt% gelatin.Abbreviation: PGA, polyglycolic acid.

Abbreviation: PGA, polyglycolic acid.

Figure 6 Scanning electron micrographs of human umbilical artery smooth muscle cells cultured on PGA/gelatin scaffolds (2000× magnification). (A) Pure PGA, (B) PGA/10 wt% gelatin, (C) PGA/30 wt% gelatin, and (D) PGA/50 wt% gelatin.Abbreviation: PGA, polyglycolic acid.

Figure 5 Scanning electron micrographs of human umbilical vein endothelial cells cultured on PGA/gelatin scaffolds (2000× magnification). (A) Pure PGA, (B) PGA/10 wt% gelatin, (C) PGA/30 wt% gelatin, and (D) PGA/50 wt% gelatin.Figure 6 Scanning electron micrographs of human umbilical artery smooth muscle cells cultured on PGA/gelatin scaffolds (2000× magnification). (A) Pure PGA, (B) PGA/10 wt% gelatin, (C) PGA/30 wt% gelatin, and (D) PGA/50 wt% gelatin.Abbreviation: PGA, polyglycolic acid.Display full sizeAbbreviation: PGA, polyglycolic acid.

Figure 6 Scanning electron micrographs of human umbilical artery smooth muscle cells cultured on PGA/gelatin scaffolds (2000× magnification). (A) Pure PGA, (B) PGA/10 wt% gelatin, (C) PGA/30 wt% gelatin, and (D) PGA/50 wt% gelatin.

Abbreviation: PGA, polyglycolic acid.

Figure 6 Scanning electron micrographs of human umbilical artery smooth muscle cells cultured on PGA/gelatin scaffolds (2000× magnification). (A) Pure PGA, (B) PGA/10 wt% gelatin, (C) PGA/30 wt% gelatin, and (D) PGA/50 wt% gelatin.Abbreviation: PGA, polyglycolic acid.