Figures & data
Figure 6 DMSAP micelles at a SPC/DMSAP ratio of 1:3 were sonicated for 10 min (A: before sonication; B: after sonication).
![Figure 6 DMSAP micelles at a SPC/DMSAP ratio of 1:3 were sonicated for 10 min (A: before sonication; B: after sonication).](/cms/asset/08a44957-fcf9-46df-ab4e-7aa3b4acaf93/dijn_a_12194646_f0006_c.jpg)
Figure 7 Size and zeta potential of micelles prepared at different ratios of SPC and DMSAP. Compared with blank and MTX-in the different ratios of SPC and DMSAP (mean ± SD; n=3).
![Figure 7 Size and zeta potential of micelles prepared at different ratios of SPC and DMSAP. Compared with blank and MTX-in the different ratios of SPC and DMSAP (mean ± SD; n=3).](/cms/asset/327c54c8-3f04-427d-acc9-ddee6d8a1939/dijn_a_12194646_f0007_c.jpg)
Figure 8 TEM of D-micelles and P/D-micelles. A: micelles self-assembled by 50mg of DMSAP in 10 mL of HEPES and after dilution with distilled water. B: Micelles formed by 75 mg of DMSAP and 25 mg SPC in the same way as A. DMSAP self-assembled to be form cylindrical micelles. Mixed lipids formed spherical micelles.
![Figure 8 TEM of D-micelles and P/D-micelles. A: micelles self-assembled by 50mg of DMSAP in 10 mL of HEPES and after dilution with distilled water. B: Micelles formed by 75 mg of DMSAP and 25 mg SPC in the same way as A. DMSAP self-assembled to be form cylindrical micelles. Mixed lipids formed spherical micelles.](/cms/asset/b3468992-5021-4230-910b-e3cf1644f171/dijn_a_12194646_f0008_c.jpg)
Figure 9 Micelles diameters were determined under different ionic strength solutions and different concentrations of BSA (A: Under different concentrations of MgSO4, Na2SO4, CaCl2 and MgCl2 in 2, 4, 8, 10, 15, 20 and 40 mM; B: Size and zeta potentials under different concentrations of BSA in 0, 2, 5, 7.5, 10 and 12.5 mM.).
![Figure 9 Micelles diameters were determined under different ionic strength solutions and different concentrations of BSA (A: Under different concentrations of MgSO4, Na2SO4, CaCl2 and MgCl2 in 2, 4, 8, 10, 15, 20 and 40 mM; B: Size and zeta potentials under different concentrations of BSA in 0, 2, 5, 7.5, 10 and 12.5 mM.).](/cms/asset/3ffbbab8-10a5-4c8b-a03d-0d47cfe705ef/dijn_a_12194646_f0009_c.jpg)
Figure 10 Efficiency of MTX entrapment in micelles using different ratios of MTX/total lipids and SPC/DMSAP. A: The ratio of MTX to total lipids was 1:5, 1:7.5, 1:10 and 1:15. B: The ratio of SPC to DMSAP was 3:1, 2:1, 1:1, 1:2 and 1:3.
![Figure 10 Efficiency of MTX entrapment in micelles using different ratios of MTX/total lipids and SPC/DMSAP. A: The ratio of MTX to total lipids was 1:5, 1:7.5, 1:10 and 1:15. B: The ratio of SPC to DMSAP was 3:1, 2:1, 1:1, 1:2 and 1:3.](/cms/asset/0bf9dd9b-69d4-4b28-acb9-149a2ee6b0ff/dijn_a_12194646_f0010_c.jpg)
Figure 11 Volume of MTX permeated across and retained in the mice skin. A: The relationship between the MTX percentage permeated across mice skin and time. B: Linear fitting of MTX volume that permeated across mice skin and time 1/2 as micelles or not as micelles. C: Relationship between the MTX percentage retained in mice skin as micelles or not as micelles.
![Figure 11 Volume of MTX permeated across and retained in the mice skin. A: The relationship between the MTX percentage permeated across mice skin and time. B: Linear fitting of MTX volume that permeated across mice skin and time 1/2 as micelles or not as micelles. C: Relationship between the MTX percentage retained in mice skin as micelles or not as micelles.](/cms/asset/67a30b09-a64e-4187-87db-eac612143806/dijn_a_12194646_f0011_c.jpg)