Figures & data
Figure 1 Influence of HTT2800 on cell viability. MESO-1 and THP-1 cells were exposed to varying concentrations of HTT2800 for 4 days. (A) Cell viability assessed by Alamar blue assay (B) Merged image of differential interference contrast and fluorescence in MESO-1 and THP-1 cells exposed to HTT2800 (nuclei were stained with H33342; bars in red indicate 20 μm).
Notes: Data are compared with dispersant medium, n = 6, mean ± standard error of the mean, *P < 0.001.
Abbreviation: DM, dispersant medium containing 0.001% gelatin.
Figure 2 DJ-1 protein expression after HTT2800 exposure. MESO-1 and THP-1 cells were exposed to varying concentrations of HTT2800 for 4 days. (A) Expression of DJ-1 protein was normalized to β-actin and represented as the ratio compared with dispersant medium (n = 4, mean ± standard error of the mean). (B) Cellular localization of DJ-1 protein in MESO-1 cells after exposure to 10 μg/mL of HTT2800 for 96 hours (DJ-1 was stained with Alexa Fluor® 594 and nuclei were stained with H33342).
Note: Bars in white indicate 10 μm.
Abbreviation: DM, dispersant medium containing 0.001% gelatin.
Figure 3 Time-course analysis of DJ-1 mRNA expression. MESO-1 cells were exposed to 10 μg/mL of HTT2800. RNA was isolated every day for 4 days; mRNA levels of DJ-1 were determined by quantitative real-time polymerase chain reaction and normalized to glyceraldehyde 3-phosphate dehydrogenase.
Notes: n = 3, mean ± standard error of the mean, *P < 0.01.
Figure 4 DJ-1 mRNA expression after exposure to HTT2800, Sumi black, and cup-stacked carbon nanotubes. MES O-1 cells were exposed to diverse carbon nanomaterials for 4 days; mRNA levels of DJ-1 were determined by quantitative real-time polymerase chain reaction and normalized to G glyceraldehyde 3-phosphate dehydrogenase.
Notes: n = 3, mean ± standard error of the mean, *P < 0.01.
Abbreviations: DM, dispersant medium containing 0.001% gelatin; SB, Sumi black; CSCNT, cup-stacked carbon nanotubes.
