Figures & data
Figure 1 The assessment of body weight and the heart coefficient ratio. (A) Body weight of all four groups of rats was recorded twice a week, which increased in a similar trend. (B) The heart coefficient ratio was calculated and analyzed. Groups with no label on the top indicate no significant difference (P>0.05) when compared among the groups.
![Figure 1 The assessment of body weight and the heart coefficient ratio. (A) Body weight of all four groups of rats was recorded twice a week, which increased in a similar trend. (B) The heart coefficient ratio was calculated and analyzed. Groups with no label on the top indicate no significant difference (P>0.05) when compared among the groups.](/cms/asset/801c0d49-02f3-49c4-b579-ce6401289238/dijn_a_12194855_f0001_b.jpg)
Table 1 Echocardiography Measurements in Rats Treated with or Without AgNPs and Selenite
Table 2 Hemodynamics Measurements in Rats Treated with or Without AgNPs and Selenite
Figure 2 Assessment of cardiac function by echocardiography and invasive hemodynamics. (A) Representative M-mode echocardiograms images were captured in each rat. (B) Representative left ventricular pressure diagrams were recorded in each group.
![Figure 2 Assessment of cardiac function by echocardiography and invasive hemodynamics. (A) Representative M-mode echocardiograms images were captured in each rat. (B) Representative left ventricular pressure diagrams were recorded in each group.](/cms/asset/af7e801e-cd18-4b77-b9ec-f63048fb170f/dijn_a_12194855_f0002_c.jpg)
Figure 3 Ultrastructural changes of heart tissue observed by 10,000×TEM. Sarcomere and myofilament were observed. After exposure of AgNPs, fragmentation and lysis of the myofibrils and disarray of the sarcomere were found.
![Figure 3 Ultrastructural changes of heart tissue observed by 10,000×TEM. Sarcomere and myofilament were observed. After exposure of AgNPs, fragmentation and lysis of the myofibrils and disarray of the sarcomere were found.](/cms/asset/729d076c-fc1d-4463-8330-4e64cafcaef4/dijn_a_12194855_f0003_b.jpg)
Figure 4 Ultrastructural changes of myocardial capillaries observed by 20,000×TEM. (A) Ultrastructural changes in myocardial capillaries and their endothelial cells. (B) The summarized bar graph showing the relative luminal area and the relative nuclear area.
![Figure 4 Ultrastructural changes of myocardial capillaries observed by 20,000×TEM. (A) Ultrastructural changes in myocardial capillaries and their endothelial cells. (B) The summarized bar graph showing the relative luminal area and the relative nuclear area.](/cms/asset/9f6b3def-5f18-4c08-bcbd-23ccd8a55dbc/dijn_a_12194855_f0004_b.jpg)
Figure 5 Ultrastructural changes of mitochondrial morphology and the mitophagy. (A–D) representative images showing mitochondrial morphology from (A) control, (B) Se-treated, (C) AgNPs-treated and (D) Se+AgNPs cotreated group; Green arrows denote silver nanoparticles within the mitochondria and yellow for outside. (E and F) representative images showing mitophagy in (E) AgNPs-treated and (F) Se+AgNPs cotreated group. Red arrows denote mitophagy.
![Figure 5 Ultrastructural changes of mitochondrial morphology and the mitophagy. (A–D) representative images showing mitochondrial morphology from (A) control, (B) Se-treated, (C) AgNPs-treated and (D) Se+AgNPs cotreated group; Green arrows denote silver nanoparticles within the mitochondria and yellow for outside. (E and F) representative images showing mitophagy in (E) AgNPs-treated and (F) Se+AgNPs cotreated group. Red arrows denote mitophagy.](/cms/asset/d5bb8501-be36-4510-ad74-31b954f7378e/dijn_a_12194855_f0005_b.jpg)
Figure 6 Western blotting of mitochondrial fission and autophagy markers. (A) The representative Western blot showing Drp1, p-Drp1, Fis1 and LC3B levels. VDAC and GAPDH served as the internal loading control for mitochondrial and whole protein, respectively. (B) The bar graph showing semiquantitative band intensity ratio from Drp1, p-Drp1, Fis1 to LC3B.
![Figure 6 Western blotting of mitochondrial fission and autophagy markers. (A) The representative Western blot showing Drp1, p-Drp1, Fis1 and LC3B levels. VDAC and GAPDH served as the internal loading control for mitochondrial and whole protein, respectively. (B) The bar graph showing semiquantitative band intensity ratio from Drp1, p-Drp1, Fis1 to LC3B.](/cms/asset/697ac2ec-5e42-4ba0-ad92-1e870d257a9b/dijn_a_12194855_f0006_b.jpg)