ROS-Generating Amine-Functionalized Magnetic Nanoparticles Coupled with Carboxymethyl Chitosan for pH-Responsive Release of Doxorubicin

Figures & data

Figure 1 ¹H-NMR spectrum of CMC.

Figure 2 XRD patterns of CMC and amine-MNPs.

Figure 3 FTIR spectra of (a) CMC, (b) amine-MNPs, (c) DOX, (d) MNPs-CMC and (e) MNPs-CMC-DOX.

Figure 4 TEM images of (A) amine-MNPs and (B) MNPs-CMC-DOX. Scale bar = 20 nm. SAED patterns of (C) amine-MNPs and (D) MNPs-CMC-DOX. Scale bar = 51 nm.

Figure 5 SEM images of (A) amine-MNPs and (B) MNPs-CMC-DOX. The size distribution of (C) amine-MNPs and (D) MNPs-CMC-DOX as determined by DLS.

Figure 6 (A) TGA and (B) DSC curves of CMC, amine-MNPs, MNPs-CMC, DOX, and MNPs-CMC-DOX.

Figure 7 Release patterns of DOX from MNPs-CMC-DOX at (A) 37°C and (B) 25°C.

Table 1 Release Kinetic Parameters of MNPs-CMC-DOX at Different pH Values (6.8 and 1.2) and Temperatures (37°C and 25°C)

pH	Temperature	Zero-Order Model		First-Order Model		Higuchi Model		Korsmeyer-Peppas Model
		K0	r^2	K1	r^2	KH	r^2	n	r^2
1.2	37°C	0.940	0.878	0.016	0.977	7.394	0.970	0.618	0.991
	25°C	0.899	0.884	0.015	0.973	7.065	0.968	0.624	0.990
6.8	37°C	0.866	0.904	0.014	0.982	6.769	0.965	0.645	0.994
	25°C	0.823	0.914	0.013	0.980	6.415	0.960	0.659	0.994

Figure 8 Viability of MCF-7 cells after treatment with (A) DOX, (B) MNPs-CMC-DOX, and (C) MNPs-CMC.

Figure 9 (A) Effect of different treatments on the intracellular ROS level of MCF-7 cells as determined by the H₂DCFDA assay: (i) no treatment, (ii) treatment with H₂O₂, (iii) treatment with DOX, (iv) treatment with MNPs-CMC, and (v) treatment MNPs-CMC-DOX. (B) Viability of 3T3 cells after treatment with DOX, MNPs-CMC-DOX, and MNPs-CMC.