Minocycline hydrochloride nanoliposomes inhibit the production of TNF-α in LPS-stimulated macrophages

Figures & data

Figure 1 Scanning electron microscopy images (A) and structure schematic drawing (B) of liposomes.

Figure 2 Effects of macrophages proliferation rate of 10–100 μg/mL 2% minocycline hydrochloride nanoliposomes, periocline, and 2% minocycline hydrochloride solution work on ANA-1 for 1, 6, 12, 18, 24, 48, and 60 hours.

Abbreviations: hr, hours; Periocline, 2% minocycline hydrochloride gel; ANA-1, murine macrophages (ANA-1).

Table 1 The influence of factors of time and concentration work on the cell relative growth rate of 2% minocycline hydrochloride nanoliposomes, periocline, and 2% minocycline hydrochloride solution

	Variable	DF	Parameter estimate	Standard error	t value	Pr > |t|
2% minocycline hydrochloride nanoliposomes	x1	1	−0.29896	0.02949	−10.14	<0.0001
	x2	1	−0.17686	0.05322	−3.32	<0.0002
	x1*x2	1	−0.00454	0.00093356	−4.87	<0.0001
Periocline	x1	1	−0.29928	0.02930	−10.22	<0.0001
	x2	1	−0.19571	0.05287	−3.70	<0.0007
	x1*x2	1	−0.00423	0.00092739	−4.56	<0.0001
2% minocycline hydrochloride solution	x1	1	−0.29288	0.02967	−9.87	<0.0001
	x2	1	−0.20907	0.05355	−3.90	<0.0004
	x1*x2	1	−0.00404	0.00093938	−4.30	<0.0001

Notes: x1, 10, 20, 40, 50, 70 and 100 μg/mL; x2, 1, 6, 12, 18, 24, 48, and 60 hours; x1*x2, reciprocal effect between concentration and time factors.

Abbreviations: Periocline, 2% minocycline hydrochloride gel; DF, degree of freedom.

Figure 3 Quantitative analysis of RT-PCR products of TNF-α mRNA at 6 hours, 12 hours, 24 hours, 48 hours and 60 hours; values are means ± SD from three duplicate experiments. *P < 0.05, different from the value in LPS treated groups; **P < 0.05, different from the value in periocline-treated groups; ^#P < 0.05, different from the value in 2% minocycline hydrochloride solution treated groups. Drug concentration of experimental group, positive control I and positive control II were 10 (A), 20 (B), 40 (C), 50 (D) and 70 (E) μg/mL respectively.

Abbreviations: Periocline, 2% minocycline hydrochloride gel; RT-PCR, Reverse Transcription-Polymerase Chain Reaction; TNF-α, Tumor necrosis factor-alpha; LPS, Lipopolysaccharide; SD, standard deviation.