Development of a Point-of-Care Test Based on Selenium Nanoparticles for Heart-Type Fatty Acid-Binding Proteins in Human Plasma and Blood

Figures & data

Figure 1 Diagram and components of the lateral flow immunoassay test strip for H-FABP (A) and visual assessment guidelines for interpreting the test strip results (B). Abbreviations: H-FABP: Heart-type fatty acid binding protein; C: Control line; T: Test line.

Figure 2 Characterization of SeNPs: (A) images of SeNPs, SeNPs after centrifugation, and SeNPs after suspension. (B) TEM result of SeNPs, (C) PDI result and (D) ZP result of SeNPs by DLS.

Abbreviations: SeNPs: Selenium nanoparticles; TEM: Transmission electron microscopy; DLS: Dynamic light scattering; PDI: Polymer dispersity index; ZP: Zeta potential.

Figure 3 Characteristion, optimal pH and labelling concentration of the SeNps-anti-H-FABP mAb1. TEM (A) and DLS (B) result of SeNps-anti-H-FABP mAb; Detection results and auxiliary judgement result (C) with H-FABP kit at different pH values; Detection results and auxiliary judgement result (D) with H-FABP kit at different concentrations of mAb1.

Abbreviations: SeNPs: Selenium nanoparticles; H-FABP: Heart-type fatty acid binding protein; mAb: Monoclonal antibody; TEM: Transmission electron microscopy; DLS: Dynamic light scattering; PDI: Polymer dispersity index; C: Control line; T: Test line.

Figure 4 The limit of detection of the H-FABP kit in plasma and blood. Detection results for H-FABP in plasma observed by the naked eyes (A) and auxiliary interpretation results by ImageJ and GraphPad Prism (B). Detection results for H-FABP in blood observed by the naked eyes (C) and auxiliary interpretation results by ImageJ and GraphPad Prism (D).

Abbreviations: H-FABP: Heart-type fatty acid binding protein; C: Control line; T: Test line.

Figure 5 The specificity and stability of the H-FABP kit. Detection results of specificity for H-FABP observed by the naked eyes (A) and auxiliary interpretation results by ImageJ and GraphPad Prism (B). Detection results of stability for H-FABP observed by the naked eyes and auxiliary interpretation results by ImageJ and GraphPad Prism (C). Abbreviations: H-FABP: Heart-type fatty acid binding protein; cTnI: Cardiac troponin I; Myo: Myoglobin; CK-MB: Creatine kinase-Mb; C: Control line; T: Test line.

Table 1 Results of Recovery Experiment for H-FABP

Category Standard (ng/mL)		Number of Experiment			Ave	SD	CV (%)
		1	2	3
H-FABP	0.5	0.4327	0.4541	0.4260	0.4376	0.01468	3.35%
	1.0	0.9323	0.9413	0.8908	1.0674	0.02694	2.92%

Abbreviations: H-FABP, heart-type fatty acid binding protein; Ave, average; SD, standard deviation; CV, coefficient of variation.

Table 2 Distribution of Detection Results for Actual Samples

Experimental Methods	Confirmed by ELISA
	Positive Samples	Negative Samples	Total Samples
Positive by Se-NPs kit	16	1	17
Negative by Se-NPs kit	0	29	29
Total	16	30	46

Abbreviations: Se-NPs, selenium nanoparticles; ELISA, enzyme-linked immunosorbent assay.

Figure 6 Detection results of actual samples of this kits for H-FABP in plasma and blood samples. Detection results of plasma for H-FABP observed by the naked eyes (A) and auxiliary interpretation results by ImageJ and GraphPad Prism (B); Detection results of blood for H-FABP observed by the naked eyes (C) and auxiliary interpretation results by ImageJ and GraphPad Prism (D).

Abbreviations: ELISA: Enzyme linked immunosorbent assay; P: Positive; N: Negative; C: Control line; T: Test line.