Figures & data
Figure 1 Diagram and components of the lateral flow immunoassay test strip for H-FABP (A) and visual assessment guidelines for interpreting the test strip results (B). Abbreviations: H-FABP: Heart-type fatty acid binding protein; C: Control line; T: Test line.
![Figure 1 Diagram and components of the lateral flow immunoassay test strip for H-FABP (A) and visual assessment guidelines for interpreting the test strip results (B). Abbreviations: H-FABP: Heart-type fatty acid binding protein; C: Control line; T: Test line.](/cms/asset/3a840882-0053-48ba-b76a-d95942e38fb9/dijn_a_12184835_f0001_c.jpg)
Figure 2 Characterization of SeNPs: (A) images of SeNPs, SeNPs after centrifugation, and SeNPs after suspension. (B) TEM result of SeNPs, (C) PDI result and (D) ZP result of SeNPs by DLS.
![Figure 2 Characterization of SeNPs: (A) images of SeNPs, SeNPs after centrifugation, and SeNPs after suspension. (B) TEM result of SeNPs, (C) PDI result and (D) ZP result of SeNPs by DLS.](/cms/asset/66c06c6f-28f1-4ea1-a5f8-941a91008e3d/dijn_a_12184835_f0002_c.jpg)
Figure 3 Characteristion, optimal pH and labelling concentration of the SeNps-anti-H-FABP mAb1. TEM (A) and DLS (B) result of SeNps-anti-H-FABP mAb; Detection results and auxiliary judgement result (C) with H-FABP kit at different pH values; Detection results and auxiliary judgement result (D) with H-FABP kit at different concentrations of mAb1.
![Figure 3 Characteristion, optimal pH and labelling concentration of the SeNps-anti-H-FABP mAb1. TEM (A) and DLS (B) result of SeNps-anti-H-FABP mAb; Detection results and auxiliary judgement result (C) with H-FABP kit at different pH values; Detection results and auxiliary judgement result (D) with H-FABP kit at different concentrations of mAb1.](/cms/asset/6d2153a1-44ab-4381-9b38-28b786307f9f/dijn_a_12184835_f0003_c.jpg)
Figure 4 The limit of detection of the H-FABP kit in plasma and blood. Detection results for H-FABP in plasma observed by the naked eyes (A) and auxiliary interpretation results by ImageJ and GraphPad Prism (B). Detection results for H-FABP in blood observed by the naked eyes (C) and auxiliary interpretation results by ImageJ and GraphPad Prism (D).
![Figure 4 The limit of detection of the H-FABP kit in plasma and blood. Detection results for H-FABP in plasma observed by the naked eyes (A) and auxiliary interpretation results by ImageJ and GraphPad Prism (B). Detection results for H-FABP in blood observed by the naked eyes (C) and auxiliary interpretation results by ImageJ and GraphPad Prism (D).](/cms/asset/f410b2cf-0857-4dbc-a6b2-97e09afcbc23/dijn_a_12184835_f0004_c.jpg)
Figure 5 The specificity and stability of the H-FABP kit. Detection results of specificity for H-FABP observed by the naked eyes (A) and auxiliary interpretation results by ImageJ and GraphPad Prism (B). Detection results of stability for H-FABP observed by the naked eyes and auxiliary interpretation results by ImageJ and GraphPad Prism (C). Abbreviations: H-FABP: Heart-type fatty acid binding protein; cTnI: Cardiac troponin I; Myo: Myoglobin; CK-MB: Creatine kinase-Mb; C: Control line; T: Test line.
![Figure 5 The specificity and stability of the H-FABP kit. Detection results of specificity for H-FABP observed by the naked eyes (A) and auxiliary interpretation results by ImageJ and GraphPad Prism (B). Detection results of stability for H-FABP observed by the naked eyes and auxiliary interpretation results by ImageJ and GraphPad Prism (C). Abbreviations: H-FABP: Heart-type fatty acid binding protein; cTnI: Cardiac troponin I; Myo: Myoglobin; CK-MB: Creatine kinase-Mb; C: Control line; T: Test line.](/cms/asset/315e4fbe-0fdb-47ce-a072-d7747df0eced/dijn_a_12184835_f0005_c.jpg)
Table 1 Results of Recovery Experiment for H-FABP
Table 2 Distribution of Detection Results for Actual Samples
Figure 6 Detection results of actual samples of this kits for H-FABP in plasma and blood samples. Detection results of plasma for H-FABP observed by the naked eyes (A) and auxiliary interpretation results by ImageJ and GraphPad Prism (B); Detection results of blood for H-FABP observed by the naked eyes (C) and auxiliary interpretation results by ImageJ and GraphPad Prism (D).
![Figure 6 Detection results of actual samples of this kits for H-FABP in plasma and blood samples. Detection results of plasma for H-FABP observed by the naked eyes (A) and auxiliary interpretation results by ImageJ and GraphPad Prism (B); Detection results of blood for H-FABP observed by the naked eyes (C) and auxiliary interpretation results by ImageJ and GraphPad Prism (D).](/cms/asset/4fb79a97-2fa1-406d-849a-6dfa26c7313d/dijn_a_12184835_f0006_c.jpg)