Figures & data
Table 1 Primer Sequences Used for Real-Time RT-PCR
Figure 1 (A) Graphic illustration of preparation mechanism of riclin, and riclin/AgNPs hydrogels; (B) UV–vis absorption spectra of aqueous dispersions of AgNPs; (C) XRD pattern of riclin hydrogels; (D) XRD pattern of riclin/AgNPs hydrogels; (E) TEM images of AgNPs; (F) Particle size distribution of AgNPs; (G) Zeta potential of the synthesized AgNPs.
![Figure 1 (A) Graphic illustration of preparation mechanism of riclin, and riclin/AgNPs hydrogels; (B) UV–vis absorption spectra of aqueous dispersions of AgNPs; (C) XRD pattern of riclin hydrogels; (D) XRD pattern of riclin/AgNPs hydrogels; (E) TEM images of AgNPs; (F) Particle size distribution of AgNPs; (G) Zeta potential of the synthesized AgNPs.](/cms/asset/ee7e5205-9501-4d8f-a6c8-4715719e9732/dijn_a_12184882_f0001_c.jpg)
Figure 2 Rheological and moisture retention properties of pristine riclin and riclin/AgNPs hydrogel. (A) Shear stress of the samples per weight as a function of the shear rate; (B) viscosity vs shear rate; (C) storage modulus (G′, solid symbols) and loss modulus (G′′, hollow symbols) as a function of the strain amplitude; (D) moisture retention curve of riclin and riclin/AgNPs hydrogel.
![Figure 2 Rheological and moisture retention properties of pristine riclin and riclin/AgNPs hydrogel. (A) Shear stress of the samples per weight as a function of the shear rate; (B) viscosity vs shear rate; (C) storage modulus (G′, solid symbols) and loss modulus (G′′, hollow symbols) as a function of the strain amplitude; (D) moisture retention curve of riclin and riclin/AgNPs hydrogel.](/cms/asset/56822ee5-a1af-4282-b1dc-a0ff207c1f87/dijn_a_12184882_f0002_c.jpg)
Figure 3 (A) In vitro release of AgNPs are compared between control (AgNPs) and treatment (riclin/AgNPs). Loading of AgNPs into hydrogel was resulted in a prolonged release pattern of the nanoparticles over 48 hours of exposure; (B) in vitro degradation of riclin/AgNPs hydrogel. (C) The pictures of riclin/AgNPs hydrogel at different degradation time.
![Figure 3 (A) In vitro release of AgNPs are compared between control (AgNPs) and treatment (riclin/AgNPs). Loading of AgNPs into hydrogel was resulted in a prolonged release pattern of the nanoparticles over 48 hours of exposure; (B) in vitro degradation of riclin/AgNPs hydrogel. (C) The pictures of riclin/AgNPs hydrogel at different degradation time.](/cms/asset/221c4d8a-9bb0-4a3f-9c7b-93767dd40b8a/dijn_a_12184882_f0003_c.jpg)
Figure 4 Antibacterial activity of pristine riclin and riclin/AgNPs hydrogels against E. coli (A) and S. aureus (B); all data are presented by the mean ± standard deviation (SD, n = 3) (p < 0.05); Viability of NIH3T3 mouse fibroblasts (C) and Raw 264.7 (D) after 24-h exposure to different concentrations of AgNPs and riclin/AgNPs composed hydrogel.
![Figure 4 Antibacterial activity of pristine riclin and riclin/AgNPs hydrogels against E. coli (A) and S. aureus (B); all data are presented by the mean ± standard deviation (SD, n = 3) (p < 0.05); Viability of NIH3T3 mouse fibroblasts (C) and Raw 264.7 (D) after 24-h exposure to different concentrations of AgNPs and riclin/AgNPs composed hydrogel.](/cms/asset/6e25c253-9b51-4592-a063-431175b711e3/dijn_a_12184882_f0004_c.jpg)
Figure 5 Effect of test samples on the healing of S. aureus-infected wounds in C57BL/6 mice. (A) Representative photographs of the healing of S. aureus-infected wounds treated with riclin or riclin/AgNPs. (B) Percentage of the area reduction of mouse skin lesions relative to the initial wound. Data are the mean ± standard deviation (n = 6); *P < 0.05, compared with the untreated group.
![Figure 5 Effect of test samples on the healing of S. aureus-infected wounds in C57BL/6 mice. (A) Representative photographs of the healing of S. aureus-infected wounds treated with riclin or riclin/AgNPs. (B) Percentage of the area reduction of mouse skin lesions relative to the initial wound. Data are the mean ± standard deviation (n = 6); *P < 0.05, compared with the untreated group.](/cms/asset/24e419f0-46c1-4ab1-b4fa-1aaf77bcd645/dijn_a_12184882_f0005_c.jpg)
Figure 6 (A) Histological (H&E and MT staining) analysis of S. aureus-infected wounds in C57BL/6 mice. Scale bar = 100 μm; H&E, hematoxylin and eosin; MT, Masson’s trichrome. Red arrows indicate blood vessels; blue arrows indicate early epithelialization, pink arrows indicate fibroblasts, and Orange arrows indicate mononuclear inflammatory cells. The blue color in the MT-stained tissue indicates collagen, arranged parallel to the skin surface. (B) Gene expression analysis of IL-6, IL-1β, TNF-α inflammatory mediators in wounds treated with hydrogels of riclin and riclin/AgNPs after 8 days of treatment. Data are presented as mean ± SD, n = 3. One-way ANOVA was used to evaluate the statistical differences, ***Represents p < 0.001.
![Figure 6 (A) Histological (H&E and MT staining) analysis of S. aureus-infected wounds in C57BL/6 mice. Scale bar = 100 μm; H&E, hematoxylin and eosin; MT, Masson’s trichrome. Red arrows indicate blood vessels; blue arrows indicate early epithelialization, pink arrows indicate fibroblasts, and Orange arrows indicate mononuclear inflammatory cells. The blue color in the MT-stained tissue indicates collagen, arranged parallel to the skin surface. (B) Gene expression analysis of IL-6, IL-1β, TNF-α inflammatory mediators in wounds treated with hydrogels of riclin and riclin/AgNPs after 8 days of treatment. Data are presented as mean ± SD, n = 3. One-way ANOVA was used to evaluate the statistical differences, ***Represents p < 0.001.](/cms/asset/98edd81e-bf41-4ebd-a928-18d6cc1779b1/dijn_a_12184882_f0006_c.jpg)