Codelivery of zoledronic acid and doublestranded RNA from core-shell nanoparticles

Figures & data

Figure 1 Fabrication of lipid-coated calcium phosphate nanoparticles.

Abbreviations: CaP, calcium phosphate; LCP, lipid-coated calcium phosphate nanoparticles; poly (I:C), polyinosinic acid-polycytidylic acid.

Figure 2 (A) Effect of poly (I:C) on particle size of LCP without zoledronic acid. (B) Effect of ratio of poly (I:C) to zoledronic acid on particle size of LCP.

Abbreviations: LCP, lipid-coated calcium phosphate nanoparticles; poly (I:C), polyinosinic acid-polycytidylic acid; zol, zoledronic acid.

Table 1 Diameter, polydispersity index, zeta potential, and encapsulation efficiency of different nanoparticles

	Diameter (nm)^Citation1	Polydispersity index	Zeta potential (mV)	Encapsulation efficiency (% zoledronic acid)
LCP-poly (I:C)	184 ± 25	0.22 ± 0.02	43.2 ± 5.3	N/A
Lipoplex-poly (I:C)-zoledronic acid^2	138 ± 40	0.33 ± 0.30	44.2 ± 6.3	42 ± 5.7
CaP-poly (I:C)-zoledronic acid^2	359 ± 15	0.12 ± 0.10	−10.1 ± 2.5	90 ± 2.4
LCP-poly (I:C)-zoledronic acid^2	205 ± 20	0.17 ± 0.04	21.7 ± 4.2	94 ± 1.3

Notes: Data are presented as the mean ± standard deviation; poly (I:C)-zoledronic acid = 1:1 (weight ratio).

Abbreviations: CaP, calcium phosphate; LCP, lipid-coated calcium phosphate nanoparticles; poly (I:C), polyinosinic acid-polycytidylic acid.

Figure 3 Size distribution of LCP-poly (I:C)-zoledronic acid.

Abbreviations: LCP, lipid-coated calcium phosphate nanoparticles; poly (I:C), polyinosinic acid-polycytidylic acid.

Figure 4 Change of particle size of LCP-poly (I:C)-zoledronic acid and CaP-poly (I:C)-zoledronic acid at 4°C within 30 days.

Abbreviations: CaP, calcium phosphate; LCP, lipid-coated calcium phosphate nanoparticles; poly (I:C), polyinosinic acid-polycytidylic acid; ZOL, zoledronic acid.

Figure 5 In vitro cytotoxicity of free poly (I:C), CaP-poly (I:C), Lipoplex-poly (I:C), and LCP-poly (I:C) in a B16BL6 cell line, with a poly (I:C) concentration of 0.5 μg/mL.

Notes: *P < 0.05, compared with free poly (I:C); **P < 0.05, compared with lipoplex-poly (I:C).

Abbreviations: CaP, calcium phosphate; LCP, lipid-coated calcium phosphate nanoparticles; poly (I:C), polyinosinic acid-polycytidylic acid.

Figure 6 In vitro cytotoxicity of LCP-poly (I:C)-zoledronic acid in B16BL6 cell line with different weight ratios of poly (I:C) to zoledronic acid.

Notes: The concentration of total drugs (poly (I:C) + zoledronic acid) was 0.5 μg/mL. *P < 0.05, compared with the ratio of 3/0; **P < 0.01, compared with the ratio of 3:0.

Abbreviations: LCP, lipid-coated calcium phosphate nanoparticles; poly (I:C), polyinosinic acid-polycytidylic acid; ZOL, zoledronic acid.

Figure 7 Tumor growth inhibition by poly (I:C) and zoledronic acid codelivered by LCP in melanoma-bearing mice.

Notes: *P < 0.05 compared with PBS; **P < 0.01 compared with PBS.

Abbreviations: LCP, lipid-coated calcium phosphate nanoparticles; poly (I:C), polyinosinic acid-polycytidylic acid; ZOL, zoledronic acid; PBS, phosphate-buffered solution.

AaproMSColemanREBone health management in patients with breast cancer: current standards and emerging strategiesBreast201221181921958673

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