Figures & data
Figure 1 (a) Mechanism of liposome extraction with TiO2-based microspheres and the plasma sample preparation procedures: (b) Sample collection and extraction; (c) Total DTX method (PPE); (d) Liposomal-encapsulated DTX method (TiO2 extraction method).
Figure 2 Characterization and validation of the TiO2-based liposome samples: (a) SEM and (b) TEM images.
Figure 3 Optimization of the conditions for liposomes separation: (a) Rate of liposome extraction of DTX liposomes as a function of the amount of TiO2; (b) pH values of the dilutions.
Table 1 Optimization of the Conditions for DTX Liposome Extraction
Table 2 Back-Calculated Concentrations of Calibration Standards for All Five Assays (All of the Concentrations are Expressed as Ng/mL)
Figure 4 Representative chromatograms of all analytes in the two assays: (a) Blank sample; (b) LLOQ (7.5 ng/mL) of encapsulated DTX; (c) LLOQ (15 ng/mL) of total DTX; (d) Rat plasma sample of encapsulated DTX; (e) Rat plasma sample of total DTX; (f) IS of encapsulated DTX; (g) IS of total DTX.
Table 3 Intra- and Inter-Run Accuracy and Precision of QC Samples for All of the Analytes in Five Assays
Table 4 Matrix Effect and Rate of Liposome Extraction for Total DTX and Encapsulated DTX in Rat Plasma (n = 5 per Assay)
Table 5 Stability of DTX Liposomes in Rat Plasma (n = 5 per Assay)
Table 6 Pharmacokinetic Parameters of DTX Liposomes in Rats (n = 5 per Group)
Table 7 Summary of Statistical Analysis for Dose-Proportionality for 1 Mg/Kg, 2 Mg/Kg, and 4 Mg/Kg of DTX Liposomes
Figure 5 Concentration-time curves for total drugs and liposomal-encapsulated drugs after intravenous administration of DTX liposomes in rat. (a) 1 mg/kg; (b) 2 mg/kg, and (c) 4 mg/kg (n = 5).
