Figures & data
Figure 1 Western blot analysis of TIMP2 expression on 293T cells.
Abbreviation: TIMP2, tissue inhibitor of metalloproteinase-2.
Figure 2 (A) Mean diameter of alginate microcapsules encapsulating 293TIMP2 cells. (B) Viability of 293TIMP2 cells in alginate microcapsules. (C) Live/dead cell staining of 293TIMP2 microcapsules. The microcapsules were cultured in Dulbecco’s Modified Eagle’s Medium containing 10% fetal bovine serum for 4 weeks. Results are expressed as the mean (standard deviation) of three separate experiments.
Abbreviation: 293TIMP2, 293T cells genetically modified to secrete tissue inhibitor of metalloproteinase-2.
Figure 3 Secretion of TIMP2 from 293TIMP2 alginate microcapsules. The extent of TIMP2 secretion was analyzed by Western blotting.
Abbreviations: TIMP2, tissue inhibitor of metalloproteinase-2; 293TIMP2, 293T cells genetically modified to secrete tissue inhibitor of metalloproteinase-2; conc, concentration.
Figure 4 Gelatin zymography. U87MG cells were cultured in 10 cm dishes (cell density 70%–80% of dish area) with Dulbecco’s Modified Eagle’s Medium (DMEM) containing 10% fetal bovine serum. U87MG cells cultured with serum-free DMEM were treated with 293E or 293TIMP2 microcapsules (>100 microcapsules).
Abbreviations: TIMP2, tissue inhibitor of metalloproteinase-2; 293TIMP2, 293T cells genetically modified to secrete tissue inhibitor of metalloproteinase-2.
Figure 5 Effect of 293E and 293TIMP2 microcapsules on invasiveness of U87MG cells. The invasion test was performed using the Matrigel® assay. U87MG cells cultured in 10 cm dishes were exposed to 293E or 293TIMP2 microcapsules (>100 microcapsules) for 2 days. Next, 5 × 106 cells were seeded into the upper chamber and the cells invading the lower surface were stained with Hemacolor®. The number of invading cells was counted in four randomly selected microscopic fields per membrane. These experiments were repeated five times and are expressed as the mean (standard deviation).
Note: **P<0.001.
Abbreviations: TIMP2, tissue inhibitor of metalloproteinase-2; 293TIMP2, 293T cells genetically modified to secrete tissue inhibitor of metalloproteinase-2.
