Binding fullerenol C₆₀(OH)₂₄ to dsDNA

Figures & data

Figure 1 Fluorescence data of C₆₀(OH)₂₄ in aqueous media. A) Fluorescence emission spectra of C60(OH)₂₄ after 5 min incubation in water, with excitation at 420 nm. B) Plot of fluorescence intensity versus [C₆₀(OH)₂₄], with excitation at 420 nm; average standard error, 3.76%.

Figure 2 Fluorescence emission spectra of fullerenol (- - -) and dsDNA (——) for different excitation wavelength. A) λ = 420 nm. B) λ = 380 nm. C) λ = 360 nm. D) λ = 340 nm. [C₆₀(OH)₂₄] = 1 × 10⁻⁴ molL⁻¹; [DNA] = 1 × 10⁻⁴ molL⁻¹.

Figure 3 Fluorescence data of C₆₀(OH)₁₄ in the presence od dsDNA. A) Fluorescence emission spectra of fullerenol (——) in the presence of dsDNA (- - -) with excitation at 420 nm, as a function of fullerenol concentration; [dsDNA] = 6.31 × 10⁻⁵ molL⁻¹. B) Plot for DNA-C₆₀(OH)₂₄ system as a function of fullerenol concentration in the range of 0.94 × 10⁻⁷ to 4.5 × 10⁻⁵ molL⁻¹; [dsDNA] = 6.31 × 10⁻⁵ molL⁻¹; average standard error, 0.17%.

Figure 4 Dependence of fluorescence intensity of C₆₀(OH)₂₄ on dsDNA concentration. A) Fluorescence spectra of fullerenol (– · –) with increasing concentration of DNA (—) with excitation at 420 nm ; B) Plot of fluorescence intensity of fullerenol as a function of [dsDNA] in the concentration range of 1.3 × 10⁻⁹ to 4.4 × 10⁻⁶ molL⁻¹; [C₆₀(OH)₂₄] = 4.5 × 10⁻⁵ molL⁻¹; average standard errors: 2.69% for 2.5E⁻⁵−5E⁻⁵ region and 10.80% for 1.3E⁻⁹−3.1E⁻⁶ region.

Figure 5 Emission spectra of C₆₀(OH)₂₄ (red line), DNA-sodium salt (blue line) and C₆₀(OH)₂₄ − DNA-sodium salt complex (green) line.

Scheme 1 Binding fullerenol C₆₀(OH)₂₄ to dsDNA. A) Binding fullerenol to the major groove of sodium salt of dsDNA. B) Binding fullerenol to the outside of the dsDNA.