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Original Research

Integration of nondegradable polystyrene and degradable gelatin in a core–sheath nanofibrous patch for pelvic reconstruction

, , , , , , & show all
Pages 3193-3201 | Published online: 24 Apr 2015

Figures & data

Figure 1 The schematic diagram of electrospinning setup for core–sheath PS/gelatin nanofibrous membrane

Abbreviation: PS, polystyrene.

Figure 1 The schematic diagram of electrospinning setup for core–sheath PS/gelatin nanofibrous membraneAbbreviation: PS, polystyrene.

Table 1 Primer sequences for qRT-PCR analysis

Figure 2 SEM images of PS (A), gelatin (B), and PS/gelatin nanofibrous membrane (C); confocal fluorescent image of FITC–gelatin/PS fiber (D).

Notes: Original magnifications: ×2,000 (AC).

Abbreviations: FITC, fluorescein isothiocyanate; PS, polystyrene; SEM, scanning electron microscopy.

Figure 2 SEM images of PS (A), gelatin (B), and PS/gelatin nanofibrous membrane (C); confocal fluorescent image of FITC–gelatin/PS fiber (D).Notes: Original magnifications: ×2,000 (A–C).Abbreviations: FITC, fluorescein isothiocyanate; PS, polystyrene; SEM, scanning electron microscopy.

Figure 3 FTIR–ATR spectra of gelatin (A), polystyrene (B), and polystyrene-modified gelatin (C).

Abbreviations: ATR, attenuated total reflection; FTIR, Fourier transform infrared spectroscopy.

Figure 3 FTIR–ATR spectra of gelatin (A), polystyrene (B), and polystyrene-modified gelatin (C).Abbreviations: ATR, attenuated total reflection; FTIR, Fourier transform infrared spectroscopy.

Figure 4 Cell growth on the surface of PS (A), gelatin (B), and PS/gelatin (C) nanofibrous membrane.

Abbreviation: PS, polystyrene.

Figure 4 Cell growth on the surface of PS (A), gelatin (B), and PS/gelatin (C) nanofibrous membrane.Abbreviation: PS, polystyrene.

Figure 5 Inflammation responses to PS/gelatin at different time points postsurgery (black arrows: giant cells; scale bar =50 μm).

Notes: 1 W, 2 W, 3 W, 4 W, and 6 W indicate 1 week, 2 weeks, 3 weeks, 4 weeks, and 6 weeks, respectively.

Abbreviation: PS, polystyrene.

Figure 5 Inflammation responses to PS/gelatin at different time points postsurgery (black arrows: giant cells; scale bar =50 μm).Notes: 1 W, 2 W, 3 W, 4 W, and 6 W indicate 1 week, 2 weeks, 3 weeks, 4 weeks, and 6 weeks, respectively.Abbreviation: PS, polystyrene.

Figure 6 T-cell responses to PS/gelatin at different time points postsurgery (black arrows: positive cells).

Abbreviations: PS, polystyrene; Th, T helper cell.

Figure 6 T-cell responses to PS/gelatin at different time points postsurgery (black arrows: positive cells).Abbreviations: PS, polystyrene; Th, T helper cell.

Figure 7 Macrophage cell responses to PS/gelatin at different time points postsurgery (black arrows: positive cells).

Abbreviation: PS, polystyrene.

Figure 7 Macrophage cell responses to PS/gelatin at different time points postsurgery (black arrows: positive cells).Abbreviation: PS, polystyrene.

Figure 8 Gene expression of IFN-γ, iNOS, IL-10, and ARG in explanted tissues at different time points.

Abbreviations: IFN, interferon; iNOS, inducible nitric oxide synthase; IL, interleukin; ARG, arginase.

Figure 8 Gene expression of IFN-γ, iNOS, IL-10, and ARG in explanted tissues at different time points.Abbreviations: IFN, interferon; iNOS, inducible nitric oxide synthase; IL, interleukin; ARG, arginase.