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Original Research

Protein immobilization onto electrochemically synthesized CoFe nanowires

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Pages 645-651 | Published online: 14 Jan 2015

Figures & data

Figure 1 Schematic representation of streptavidin-Cy3 protein immobilization on CoFe nanowires.

Abbreviations: APTES, aminopropyltriethoxysilane; EDC, ethyl (dimethylaminopropyl) carbodiimide; NHS, N-hydroxysuccinimide; PBS, phosphate-buffer saline.

Figure 1 Schematic representation of streptavidin-Cy3 protein immobilization on CoFe nanowires.Abbreviations: APTES, aminopropyltriethoxysilane; EDC, ethyl (dimethylaminopropyl) carbodiimide; NHS, N-hydroxysuccinimide; PBS, phosphate-buffer saline.

Figure 2 FE-SEM image of dispersed CoFe nanowires and EDS spectrum of CoFe nanowires.

Notes: (A) FE-SEM image. (B) EDS spectrum.

Abbreviations: EDS, energy dispersive spectroscopy; FE-SEM, field emission scanning electron microscopy.

Figure 2 FE-SEM image of dispersed CoFe nanowires and EDS spectrum of CoFe nanowires.Notes: (A) FE-SEM image. (B) EDS spectrum.Abbreviations: EDS, energy dispersive spectroscopy; FE-SEM, field emission scanning electron microscopy.

Table 1 The magnetic properties and composition of CoFe nanowires by varying the source concentration of Co and Fe in electrolyte bath

Figure 3 Magnetic properties of CoFe nanowires with different ratios of Co and Fe.

Figure 3 Magnetic properties of CoFe nanowires with different ratios of Co and Fe.

Figure 4 Bright-field fluorescence images of nanowires.

Notes: (A) Bright-field image of nanowires treated with APTES and streptavidin-Cy3; (B) fluorescence image of (A); (C) bright-field image of nanowires without treating with APTES; (D) fluorescence image of (C) after treating with streptavidin-Cy3.

Abbreviation: APTES, aminopropyltriethoxysilane.

Figure 4 Bright-field fluorescence images of nanowires.Notes: (A) Bright-field image of nanowires treated with APTES and streptavidin-Cy3; (B) fluorescence image of (A); (C) bright-field image of nanowires without treating with APTES; (D) fluorescence image of (C) after treating with streptavidin-Cy3.Abbreviation: APTES, aminopropyltriethoxysilane.

Figure 5 Field emission-transmission electron microscopy images of nanowires.

Notes: (A) Streptavidin-Cy3 protein-immobilized nanowire surfaces. (B) Bare CoFe nanowire surface.

Figure 5 Field emission-transmission electron microscopy images of nanowires.Notes: (A) Streptavidin-Cy3 protein-immobilized nanowire surfaces. (B) Bare CoFe nanowire surface.

Figure 6 FT-IR spectra.

Notes: FT-IR spectra of (A) nanowires and (B) nanowires immobilized with streptavidin-Cy3 protein.

Figure 6 FT-IR spectra.Notes: FT-IR spectra of (A) nanowires and (B) nanowires immobilized with streptavidin-Cy3 protein.