Figures & data
Figure 1 TEM images of Li-MBG.
Abbreviations: TEM, transmission electron microscope; Li-MBG, Li-containing mesoporous bioglass.
Figure 2 SEM images of l-MBPC (A and B) and l-BPC (C and D) scaffolds.
Note: Arrow indicates BG particles.
Abbreviations: SEM, scanning electron microscopy; l-MBPC, Li-containing mesoporous bioglass/mPEG-PLGA-b-PLL composite; l-BPC, Li-containing bioglass/mPEG-PLGA-b-PLL composite; BG, bioglass.
Figure 3 Changes in water absorption of l-MBPC and l-BPC scaffolds with time.
Abbreviations: l-MBPC, Li-containing mesoporous bioglass/mPEG-PLGA-b-PLL composite; l-BPC, Li-containing bioglass/mPEG-PLGA-b-PLL composite.
Figure 4 Weight loss of l-MBPC and l-BPC scaffolds into PBS.
Abbreviations: l-MBPC, Li-containing mesoporous bioglass/mPEG-PLGA-b-PLL composite; l-BPC, Li-containing bioglass/mPEG-PLGA-b-PLL composite; PBS, phosphate-buffered saline.
Figure 5 SEM images of surface morphology of l-MBPC (A) and l-BPC (B) scaffolds after soaking in SBF for 7 days.
Abbreviations: SEM, scanning electron microscope; l-MBPC, Li-containing mesoporous bioglass/mPEG-PLGA-b-PLL composite; l-BPC, Li-containing bioglass/mPEG-PLGA-b-PLL composite; SBF, simulated body fluid.
Figure 6 EDS of l-MBPC (A) and l-BPC (B) scaffolds after soaking in SBF for 7 days.
Abbreviations: EDS, energy-dispersive X-ray spectroscopy; l-MBPC, Li-containing mesoporous bioglass/mPEG-PLGA-b-PLL composite; l-BPC, Li-containing bioglass/mPEG-PLGA-b-PLL composite; SBF, simulated body fluid.
Figure 7 Changes in Ca, Si, and P ion concentrations in solution after l-MBPC (A) and l-BPC (B) scaffolds immersed into SBF at different time points.
Abbreviations: l-MBPC, Li-containing mesoporous bioglass/mPEG-PLGA-b-PLL composite; l-BPC, Li-containing bioglass/mPEG-PLGA-b-PLL composite; SBF, simulated body fluid.
Figure 8 Attachment of MC3T3-E1 cells on l-MBPC, l-BPC scaffolds, and TCP at 4 hours.
Abbreviations: l-MBPC, Li-containing mesoporous bioglass/mPEG-PLGA-b-PLL composite; l-BPC, Li-containing bioglass/mPEG-PLGA-b-PLL composite; TCP, tissue culture plate.
Figure 9 Proliferation of MC3T3-E1 cells cultivated on l-MBPC and l-BPC scaffolds for 1 day, 4 days, and 7 days.
Note: The data represent the mean ± standard deviation (n=5).
Abbreviations: l-MBPC, Li-containing mesoporous bioglass/mPEG-PLGA-b-PLL composite; l-BPC, Li-containing bioglass/mPEG-PLGA-b-PLL composite; OD, mean absorbance values.
Figure 10 Cytoskeletal morphology of MC3T3-E1 cells cultivated on l-MBPC (A) and l-BPC (B) scaffolds for 4 days.
Abbreviations: l-MBPC, Li-containing mesoporous bioglass/mPEG-PLGA-b-PLL composite; l-BPC, Li-containing bioglass/mPEG-PLGA-b-PLL composite.
Figure 11 ALP of MC3T3-E1 cells cultivated on l-MBPC and l-BPC scaffolds for 7 days and 10 days.
Note: The data represent the mean ± standard deviation (n=5).
Abbreviations: ALP, alkaline phosphatase; l-MBPC, Li-containing mesoporous bioglass/mPEG-PLGA-b-PLL composite; l-BPC, Li-containing bioglass/mPEG-PLGA-b-PLL composite; OD, mean absorbance values.
Figure 12 Histological evaluation (H&E staining) of materials degradation and new bone formation after implanting l-MBPC (A–C) and l-BPC (D–F) scaffolds in vivo for 1 month, 2 months, and 3 months, respectively.
Notes: m represents materials (l-MBPC: A–C and l-BPC: D–F) and b represents new bone.
Abbreviations: H&E, hematoxylin and eosin; l-MBPC, Li-containing mesoporous bioglass/mPEG-PLGA-b-PLL composite; l-BPC, Li-containing bioglass/mPEG-PLGA-b-PLL composite.
Figure 13 Quantitative analysis of the new bone area by histological observation after implanting l-MBPC and l-BPC scaffolds in vivo for 1 month, 2 months, and 3 months.
Abbreviations: l-MBPC, Li-containing mesoporous bioglass/mPEG-PLGA-b-PLL composite; l-BPC, Li-containing bioglass/mPEG-PLGA-b-PLL composite.
