Figures & data
Figure 1 Size and morphology of gold nanoparticles. The ultraviolet spectra of gold nanoparticles and transmission electron microscopic analysis (figure inset).
Note: Gold nanoparticles were synthesized following the procedure described by Turkevich et al.Citation30
![Figure 1 Size and morphology of gold nanoparticles. The ultraviolet spectra of gold nanoparticles and transmission electron microscopic analysis (figure inset).Note: Gold nanoparticles were synthesized following the procedure described by Turkevich et al.Citation30](/cms/asset/f26517e6-92f9-4e59-a167-1f42684f03a3/dijn_a_12184862_f0001_c.jpg)
Figure 2 Body weight changes for mice treated with gold nanoparticles at doses of 137.5–2200 μg/kg.
Note: Body weight was measured every two days. Each point represents the mean ± standard deviation of six mice. Data were analyzed by Student’s t-test and the differences between the doses and control group for each organ are not significant (P > 5%).
![Figure 2 Body weight changes for mice treated with gold nanoparticles at doses of 137.5–2200 μg/kg.Note: Body weight was measured every two days. Each point represents the mean ± standard deviation of six mice. Data were analyzed by Student’s t-test and the differences between the doses and control group for each organ are not significant (P > 5%).](/cms/asset/603f2466-1a61-41b8-ba54-5110e44d2b9b/dijn_a_12184862_f0002_c.jpg)
Figure 3 Body weight changes in mice treated with gold nanoparticles 1100 μg/kg by using three administration routes, ie, oral, intraperitoneal, and tail vein injection.
Note: Each point represents mean ± standard deviation. Data were analyzed by Student’s t-test. *Represents significant difference from the control group (P < 0.05).
![Figure 3 Body weight changes in mice treated with gold nanoparticles 1100 μg/kg by using three administration routes, ie, oral, intraperitoneal, and tail vein injection.Note: Each point represents mean ± standard deviation. Data were analyzed by Student’s t-test. *Represents significant difference from the control group (P < 0.05).](/cms/asset/7ef540e7-a1c0-4cb9-8636-a2bed0c7ff99/dijn_a_12184862_f0003_c.jpg)
Table 1 A summary of in vivo toxicity of gold nanoparticles
Figure 4 Transmission electron microscopy figures for gold nanoparticles in bone marrow and blood cells 14 days after oral administration at 2200 μg/kg.
![Figure 4 Transmission electron microscopy figures for gold nanoparticles in bone marrow and blood cells 14 days after oral administration at 2200 μg/kg.](/cms/asset/69e4f473-fa1e-482c-9674-80caf69b9f9b/dijn_a_12184862_f0004_b.jpg)
Figure 5 Thymus and spleen indices of mice after oral administration by different doses (137.5–2200 μg/kg) (A) after 14 days and the different injection routes at the dose of 1100 μg/kg (B) after 28 days.
Note: All values are reported as means ± standard deviation. Data were analyzed by Student’s t- test. *Represents significant difference from the control group (P < 0.05).
Abbreviations: ORAL, oral administration; INTER, intraperitoneal injection; TAIL, tail vein injection.
![Figure 5 Thymus and spleen indices of mice after oral administration by different doses (137.5–2200 μg/kg) (A) after 14 days and the different injection routes at the dose of 1100 μg/kg (B) after 28 days.Note: All values are reported as means ± standard deviation. Data were analyzed by Student’s t- test. *Represents significant difference from the control group (P < 0.05).Abbreviations: ORAL, oral administration; INTER, intraperitoneal injection; TAIL, tail vein injection.](/cms/asset/87e2211f-ff9e-4eef-87fa-210baa00bbb1/dijn_a_12184862_f0005_c.jpg)
Table 2 Weight of organs for liver, lung, spleen, kidneys, brain, heart, and thymus after oral administration of different doses (137.5–2200 μg/kg) for 14 days
Table 3 Weight of organs for liver, lung, spleen, kidneys, brain, heart, and thymus by using oral, intraperitoneal, and tail vein injection at doses of 1100 μg/kg at 28 days
Figure 6a Hematology results from mice treated with gold nanoparticles and the control group after 14 days oral administration by different doses (137.5–2200 μg/kg).
Note: Bars represent mean ± standard deviation. Data were analyzed by Student’s t-test.
*Represents significant difference from the control group (P < 0.05).
Abbreviations: PLT, platelets; HCT, hematocrit; HGB, hemoglobin; RBC, red blood cells; WBC, white blood cells.
![Figure 6a Hematology results from mice treated with gold nanoparticles and the control group after 14 days oral administration by different doses (137.5–2200 μg/kg).Note: Bars represent mean ± standard deviation. Data were analyzed by Student’s t-test.*Represents significant difference from the control group (P < 0.05).Abbreviations: PLT, platelets; HCT, hematocrit; HGB, hemoglobin; RBC, red blood cells; WBC, white blood cells.](/cms/asset/68276b65-be62-4a2a-837b-b36f878ef666/dijn_a_12184862_f0006_c.jpg)
Figure 6b Hematology results from mice treated with gold nanoparticles and control groups after 28 days oral administration, intraperitoneal injection, and tail vein injection at the dose of 1100 μg/kg.
Note: Bars represent mean ± standard deviation. Data were analyzed by Student’s t-test.
*Represents significant difference from the control group (P < 0.05).
Abbreviations: PLT, platelets; HCT, hematocrit; HGB, hemoglobin; RBC, red blood cells; WBC, white blood cells; O/C, oral administration control; I/C, intraperitoneal injection/control; T/C, tail vein injection/control.
![Figure 6b Hematology results from mice treated with gold nanoparticles and control groups after 28 days oral administration, intraperitoneal injection, and tail vein injection at the dose of 1100 μg/kg.Note: Bars represent mean ± standard deviation. Data were analyzed by Student’s t-test.*Represents significant difference from the control group (P < 0.05).Abbreviations: PLT, platelets; HCT, hematocrit; HGB, hemoglobin; RBC, red blood cells; WBC, white blood cells; O/C, oral administration control; I/C, intraperitoneal injection/control; T/C, tail vein injection/control.](/cms/asset/02971b43-cec7-41c7-9c0d-69bffb51ef15/dijn_a_12184862_f0007_c.jpg)