Effect of Fe₃O₄-magnetic nanoparticles on acute exercise enhanced KCNQ₁ expression in mouse cardiac muscle

Figures & data

Table 1 Number of mice in each group for the study of the MNPs-Fe₃O₄

Groups		Treated with saline (n)	Treated with MNPs-Fe3O4 (n)
Non-exercised control group		6	6
Exercise groups	Euthanized at 3PE	6	6
	Euthanized at 12PE	6	6
	Euthanized at 24PE	6	6

Abbreviation: PE, post exercise.

Figure 1 Effect of a single bout of exercise on the transcription of KCNQ₁ mRNA.

Notes: All values are reported as means ± SE (n = 6). *P < 0.05, the expression levels of KCNQ₁ at 3PE, 6PE and 12PE were significantly different from the basal group.

Abbreviation: PE, post exercise.

Figure 2 Effect of a single bout of exercise on the expression of KCNQ₁ protein.

Notes: All values are reported as means ± SE (n = 6). *P < 0.05, the expression level of KCNQ₁ at 12PE was significantly different from the basal group.

Abbreviation: PE, post exercise.

Figure 3 Effect of Fe₃O₄-magnetic nanoparticles on the transcription of acute exercise enhanced KCNQ₁ mRNA in mouse cardiac muscle.

Notes: All values are reported as means ± SE (n = 6). *P < 0.05, the transcriptions level of KCNQ₁ at 3PE and 12PE in saline mouse was significantly different from the basal group. ^#P < 0.05, the transcriptions level of KCNQ₁ at 3PE and 12PE in mouse treated with MNPs-Fe₃O₄ were significantly different from the basal group.

Abbreviation: PE, post exercise.

Figure 4 Effect of Fe₃O₄-magnetic nanoparticles on the expression of acute exercise enhanced KCNQ₁ protein in mouse cardiac muscle.

Notes: All values are reported as means ± SE (n = 6). *P < 0.05, the expression level of KCNQ₁ at 12PE in saline mouse was significantly different from the basal group. ^#P < 0.05, the expression level of KCNQ₁ protein at 12PE in mouse treated with MNPs-Fe₃O₄ was significantly different from the basal group.

Abbreviation: PE, post exercise.

Table 2 Effect of MNPs-Fe₃O₄ on change of KCNQ₁ expression in mouse cardiac muscle

Group	Change of KCNQ1 mRNA (%)			Change of KCNQ1 protein (%)
	Saline	MNPs-Fe3O4	P value	Saline	MNPs-Fe3O4	P value
3PE	164 ± 24	151 ± 14	0.64	129 ± 11	136 ± 21	0.80
12PE	159 ± 23	147 ± 12	0.66	161 ± 27	152 ± 14	0.76
24PE	119 ± 17	110 ± 10	0.67	112 ± 9	116 ± 15	0.84

Notes: All values of change are compared to corresponding basal and are reported as means ± SE (n = 6). P > 0.05. P values are for comparison of MNPs-Fe₃O₄ group with corresponding saline group.

Abbreviations:SE, standard error; PE, post exercise.