Release profile and stability evaluation of optimized chitosan/alginate nanoparticles as EGFR antisense vector

Figures & data

Table 1 Nanoparticle size and zeta potential before and after the freeze-drying process

		Nanoparticles size (nm)	Zeta potential (mV)	Burst release % (up to 15 min)	T 100% (h)
Alg/Chi-AS	BFDa	196 ± 12	30.0 ± 4.1	58.3 ± 2.3	50.0 ± 0.5
N/P 5	AFDb	185 ± 8	25.0 ± 5.3	56.5 ± 2.7	51.0 ± 1.0
Alg/Chi-AS	BFD	430 ± 21	43.0 ± 3.3	50.6 ± 1.6	45.0 ± 0.7
N/P 25	AFD	425 ± 26	46.0 ± 2.7	51.3 ± 2.1	43.0 ± 1.2

Notes:

a Before freeze-drying.

b After freeze-drying.

Figure 1 Scanning electron microscopy image of alginate-chitosan nanoparticles with an N/P ratio of 5 before (A) and after (B) the freeze-drying process, magnification 50×.

Figure 2 Release profile of nanoparticles with N/P ratio of 5 and 25 (A) over 50 hours and (B) in the first six hours.

Figure 3 Gel electrophoresis analysis of alginate-chitosan nanoparticles. L (ladder), 1 (naked AS), 2 (N/P 5, one hour), 3 (N/P 5, three hours), 4 (N/P 5, three hours), 5 (N/P 25, one hour), 6 (N/P 25, three hours), 7 (N/P 25, six hours). The image has been taken after staining by ethidium bromide and visualized under ultraviolet light.

Figure 4 Swellability of antisense-loaded alginate-chitosan nanoparticles with an N/P ratio of 5, in phosphate-buffered saline at pH 7.4.