Chlamydia trachomatis infection in primary fallopian tube and high-grade serous ovarian cancers: a pilot study

Figures & data

Table 1 Demographic data, Chlamydia PCR DNA expression, and statistical analysis of the studied cases

Variant	Ovarian carcinoma N=30	Tubal adenocarcinoma N=25	Control cases N=12
Age (years), mean ± SD	54±8	59±5	58±12
P	0.06
PCR results
Positive Chlamydia PCR DNA Expression	4 (13.3%)	21 (84%)	2 (16.7%)
Negative Chlamydia PCR DNA Expression	26 (86.7%)	4 (16%)	10 (83.3%)
P^1 (χ^2)	<0.0005 (31.7)
P^2 (χ^2)	<0.0005 (27.5)
P^3 (χ^2)	<0.0005 (15.6)
P^4 (χ^2)	0.7 (0.77)

Notes: P: ANOVA test, insignificant; P¹: comparing the three groups, significant; P²: comparing ovarian vs tubal carcinoma, significant; P³: comparing tubal cancer vs control, significant; P⁴: comparing ovarian carcinoma vs control, insignificant. Bold values indicate statistical significance.

Figure 1 ROC curve analysis to calculate the best cutoff value to differentiate tubal carcinoma group vs ovarian carcinoma and control groups.

Notes: Area under the curve =0.8, P=0.01 at a cutoff value of 15.1 with sensitivity =80%, specificity =93.7%, and accuracy =88.46%; positive predictive value =77.42; negative predictive value =94.7%. The arrow points to the best cutoff value.
Abbreviation: ROC, receiver operating characteristic.

Table 2 Comparison between the three groups regarding PCR quantitative values

	Number	Mean	SD	SE	95% CI for mean		Min	Max
					Lower bound	Upper bound
Ovarian	30	13.54	71.48	13.05	−13.15	40.23	0	391.77
Tubal	25	256.69	479.84	95.97	58.63	454.76	0	1,478.84
Control	12	0.28	0.64	0.19	−0.13	0.68	0	1.72
P^1	<0.0005
P^2	<0.0005
P^3	<0.0005
P^4	0.88

Notes: P¹: Kruskal–Wallis test, significant; P²: Mann–Whitney U test comparing ovarian vs tubal carcinoma, significant; P³: Mann–Whitney U test comparing tubal carcinoma vs control, significant; P⁴: Mann–Whitney U test comparing ovarian carcinoma vs control, insignificant. Bold values indicate statistical significance.

Abbreviations: SE, standard mean of error; Min, minimum; Max, maximum.

Figure S1 High-grade serous tubal carcinoma is seen distending the tubal lumen (H&E ×10).

Figure S2 High-grade serous tubal carcinoma arises from tubal mucosa and infiltrates the submucosa (H&E ×10).