Hormonal Receptors, Human Epidermal Growth Factor Receptor-2 and Triple Negative Immunohistochemical Typing in Women with Breast Cancer in Kampala, Uganda

Figures & data

Table 1 Clinicopathologic Characteristics of the Patients (n = 103)

Variables	Frequency (n)	Percentage (%)
Age (Years)
˂41	36	35.0
41–50	30	29.1
>50	37	35.9
Histological type
Invasive carcinoma of not specified type (NST)	71	68.9
Invasive lobular carcinoma	23	22.3
Invasive micropapillary carcinoma	4	3.9
Mucinous carcinoma	3	2.9
Tubular carcinoma	1	1.0
Ductal carcinoma in situ	1	1.0
Bloom-Richardson grading system
Grade 1	16	15.5
Grade 2	55	53.4
Grade 3	32	31.1

Figure 1 Histological grade of cancers among the different age groups of the patients.

Table 2 Expression of ER, PR, and Co-Expression of ER and PR According to Age, Histological Type and Tumour Grade

Variables	ER+ (n = 55)	ER- (n = 48)	PR+ (n = 48)	PR- (n = 55)	ER+ and PR+ (n = 44)	ER- and PR- (n = 59)
Age (Years)
˂41	13(23.6)	22(45.8)	10(20.8)	25(45.5)	7(15.9)	28(47.5)
41–50	15(27.3)	16(33.3)	13(27.1)	18(32.7)	13(29.5)	18(30.5)
>50	27(49.1)	10(20.8)	25(52.1)	12(21.8)	24(54.5)	13(22.0)
P-value	0.007		0.003		0.000
95% CI	0.081–0.594		0.070–0.525		0.047–0.394
Histological type
IDC-NST	38(69.1)	33(68.8)	31(64.6)	40(72.7)	30(68.2)	41(69.5)
ILC	11(20.0)	12(25.0)	13(27.1)	10(18.2)	10(22.7)	13(22.0)
Others	6(10.9)	3(6.3)	4(8.3)	5(9.1)	4(9.1)	5(8.5)
P-value	0.630		0.643		0.773
95% CI	0.528–2.812		0.356–1.893		0.486–2.643
B-R grading
Grade 1	9(16.4)	7(14.6)	8(16.7)	8(14.5)	8(18.2)	8(13.6)
Grade 2	29(52.7)	26(54.2)	28(58.3)	27(49.1)	25(56.8)	30(50.8)
Grade 3	17(30.9)	15(31.3)	12(25.0)	20(36.4)	11(25.0)	21(35.6)
P-value	0.969		0.461		0.490
95% CI	0.10–1.0		0.491–1.510		0.473–1.493

Abbreviations: ER, estrogen receptor; PR, progesterone receptor; B-R, Bloom-Richardson grading system; IDC-NST, infiltrating ductal carcinoma-not specified type; ILC, invasive lobular carcinoma; CI, confidence interval; P, P-value.

Figure 2 (A) Intranuclear diffuse staining of the tumour cells with ER in a case of IDC-NST (IHC staining, x40), (B) Intranuclear diffuse staining of the tumour cells with PR in a case of IDC-NST (IHC staining, x40), (C) Complete nuclear membrane staining of the tumour cells with HER2 protein in a case of IDC-NST (IHC staining, x40).

Table 3 Expression of HER2 and TNBC According to Age, Histological Type and Tumour Grade

Variables	HER2+ (n = 19)	HER2- (n = 84)	TNBC (n = 39)	Not TNBC (n = 64)
Age (Years)
< 41	3(15.8)	32(38.1)	17(43.6)	18(28.1)
41–50	10(52.6)	21(25.0)	13(33.3)	18(28.1)
>50	6(31.6)	31(36.9)	9(23.1)	28(43.8)
P value	0.042		0.090
95% CI	1.011–2.109		0.789–6.331
Histological type
IDC-NST	12(63.2)	59(70.2)	29(74.4)	42(65.6)
ILC	7(36.8)	16(19.0)	7(17.9)	16(25.0)
Others	0(0.0)	9(10.7)	3(7.7)	6(9.4)
P value	0.114		0.624
95% CI	0.332–2.838		0.520–2.970
B-R grading
1	1(5.3)	15(17.9)	7(17.9)	9(14.1)
2	10(52.6)	45(53.6)	22(56.4)	33(51.6)
3	8(42.1)	24(28.6)	10(25.6)	22(34.4)
P value	0.287		0.626
95% CI	0.319–2.338		0.631–4.649

Abbreviations: HER-2, human epidermal receptor-2; TNBC, triple negative breast cancer; B-R, Bloom-Richardson grading system; IDC-NST, infiltrating ductal carcinoma-not specified type; ILC, invasive lobular carcinoma; CI, confidence interval; P, P-value.

Table 4 Univariate and Multivariate Analyses of the Association Between ER Expression and Age, Tumour Grade and Histological Types of Breast Cancer in the Patients

	ER Expression		Univariate Analysis		Multivariate Analysis
Variables	Yes (n = 55)	No (n = 48)	COR (95% CI)	P	AOR (95% CI)		P
Age group (Years)
≤50	28(50.9)	38(79.2)	1
>50	27(47.1)	10(20.8)	3.66 (1.53–8.79)	0.004	0.18 (0.062–0.541)		0.002
B-R grading
Grade 1	9(16.4)	7(14.6)	1
Grade 2	30(54.6)	25(52.1)	0.93 (0.30–2.86)	0.904	-		-
Grade 3	16(29.1)	16(33.3)	0.78 (0.23–2.60)	0.680	-		-
Histological type
IDC-NST	38(69.1)	33(68.8)	1
ILC	11(20.0)	12(25.0)	0.58(0.133–2.485)	0.459	-		-
Others	6(10.9)	3(6.3)	0.46(0.092–2.292)	0.342	-		-

Abbreviations: ER, estrogen receptor; COR, crude odds ratio; AOR, adjusted odds ratio; B-R, Bloom-Richardson grading system; IDC-NST, infiltrating ductal carcinoma-not specified type; ILC, invasive lobular carcinoma; P, P-value.

Table 5 Univariate and Multivariate Analyses for the Association Between PR Expression and Age, Tumour Grade and Histological Types of Breast Cancer in the Patients

	PR Expression		Univariate Analysis		Multivariate Analysis
Variables	Yes (n = 48)	No (n = 55)	COR (95% CI)	P	AOR (95% CI)	P
Age group (Years)
≤50	23(47.9)	43(78.2)	1
>50	25(52.1)	12(21.8)	3.89 (1.66–9.15)	0.002	0.35 (0.129–0.968)	0.043
B-R grading
Grade 1	8(16.7)	8(14.6)	1
Grade 2	28(58.3)	27(49.1)	1.04 (0.344–3.316)	0.949	-	-
Grade 3	12(25.0)	20(36.4)	0.60 (0.18–2.02)	0.409	-	-
Histological type
IDC-NST	31(64.6)	40(72.7)	1
ILC	13(27.1)	10(18.2)	0.97 (0.240–3.913)	0.964	-	-
Others	4(8.3)	5(9.1)	1.63 (0.344–7.670)	0.540	-	-

Abbreviations: PR, progesterone receptor; COR, crude odds ratio; AOR, adjusted odds ratio; B-R, Bloom-Richardson grading system; IDC-NST, infiltrating ductal carcinoma-not specified type; ILC, invasive lobular carcinoma; P, P-value.

Table 6 Univariate and Multivariate Analyses for the Association of Co-Expression of ER and PR and Age, Tumour Grade and Histological Types of Breast Cancer in the Patients

	ER and PR Co-Expression		Univariate Analysis		Multivariate Analysis
Variables	Yes (n = 44)	No (n = 59)	COR (95% CI)	P	AOR (95% CI)	P
Age group (Years)
≤50	20(45.5)	45(76.3)	1
>50	24(54.5)	14(23.7)	3.86(1.659–8.968)	0.001	0.86(0.142–1.051)	0.063
B-R grading
Grade 1	8(18.2)	8(13.6)	1
Grade 2	25(56.8)	30(50.8)	1.91(0.563–6.477)	0.300	-	-
Grade 3	11(25.0)	21(35.6)	1.59(0.645.3.921)	0.313	-	-
Histological type
IDC-NST	30(68.2)	41(69.5)	1
ILC	10(22.7)	13(22.0)	0.92(0.226–3.696)	0.900	-	-
Others	4(9.1)	5(8.5)	0.96(0.204–4.539)	0.960	-	-

Abbreviations: ER and PR, estrogen receptor and progesterone receptor; COR, crude odds ratio; AOR, adjusted odds ratio; B-R, Bloom-Richardson grading system; IDC-NST, infiltrating ductal carcinoma-not specified type; ILC, invasive lobular carcinoma; P, P-value.

Table 7 Univariate and Multivariate Analyses for the Association Between HER2 Expression and Age, Tumour Grade and Histological Types of Breast Cancer in the Patients

	HER2 Expression		Univariate Analysis		Multivariate Analysis
Variables	Yes (n = 19)	No (n = 84)	COR (95% CI	P	AOR (95% CI		P
Age group (Years)
≤50	13(68.4)	53(63.1)	1
>50	6(31.6)	31(36.9)	0.79(0.27–2.29)	0.663	–		–
B-R grading
Grade 1	1(5.3)	15(17.9)	1
Grade 2	10(52.6)	45(53.6)	3.33(0.39–28.25)	0.27	0.10(0.010–1.048)		0.055
Grade 3	8(42.1)	24(28.6)	5.0(0.57–44.08)	0.147	0.48(0.141–1.613)		0.233
Histological type
IDC-NST	12(63.2)	59(70.2)	1
ILC	7(36.8)	16(19.0)	0.72(0.54–9.02)	0.116	0.33(0.401–11.63)		0.713
Others	0(0.0)	9(10.7)	1.03(0.35–3.01)	0.957	-		-

Abbreviations: HER2, human-epidermal receptor-2; COR, crude odds ratio; AOR, adjusted odds ratio; B-R, Bloom-Richardson grading system; IDC-NST, infiltrating ductal carcinoma-not specified type; ILC, invasive lobular carcinoma; P, P-value.

Table 8 Univariate and Multivariate Analyses for the Association Between TNBC and Age, Tumour Grade and Histological Types of Breast Cancer in the Patients

	TNBC Expression		Univariate Analysis		Multivariate Analysis
Variables	Yes (n = 39)	No (n = 64)	COR (95% CI)	P	AOR (95% CI)	P
Age group (Years)
≤50	30(76.9)	36(56.3)	1
>50	9(23.1)	28(43.8)	0.39(0.16–0.94)	0.037	4.38(1.451–13.223)	0.009
B-R grade
Grade 1	7(18.0)	9(14.1)	1
Grade 2	21(53.9)	34(53.1)	0.79(0.26–2.45)	0.69	-	-
Grade 3	11(28.2)	21(32.8)	0.67(0.20–2.30)	0.53	-	-
Histological type
IDC-NST	29(74.4)	42(65.6)	1
ILC	7(19.9)	16(25.0)	0.4(0.21–3.51)	0.31	-
Others	3(7.7)	6(9.4)	0.8(0.34–1.92)	0.62	-	-

Abbreviations: TNBC, triple negative breast cancer; COR, crude odds ratio; AOR, adjusted odds ratio; B-R, Bloom-Richardson grading system; IDC-NST, infiltrating ductal carcinoma-not specified type; ILC, invasive lobular carcinoma; P, P-value.