Assisted Reproductive Technology Outcomes in Women with Normal Ovarian Response Receiving Recombinant Luteinizing Hormone/Human Menopausal Gonadotropin: An Observational Study

Figures & data

Table 1 Characteristics of Women with Normal Ovarian Response Who Received the IVF-ET

Variables	Group I (n=115)	Group II (n=244)	Group III (n=97)	P
Age (years), mean±SD	30.90±3.75	31.43±3.86	32.38±4.24^ab*	0.023
BMI (kg/m^2), mean±SD	22.04±3.11	21.78±3.14	23.61±3.34^ab**	<0.001
Type of infertility, n (%)
Primary	46 (40.0)	113 (49.1)	19 (38.8)
Secondary	69 (60.0)	117 (50.9)	30 (61.2)
Baseline FSH (U/L), mean±SD	6.58±1.56	6.82±1.53	6.69±1.53	0.386
Baseline LH (U/L), mean±SD	4.51±2.18	4.75±2.41	4.98±2.46	0.362
Baseline FSH/LH ratio, mean±SD	1.67±0.60	1.66±0.60	1.58±0.62	0.512
Baseline prolactin (ng/mL), mean±SD	13.94±8.34	13.77±9.03	12.20±7.20	0.282
Baseline progesterone (ng/mL), mean±SD	0.54±0.33	0.69±1.52	0.95±2.10	0.214
Baseline estradiol (pg/mL), mean±SD	52.96±56.03	52.02±38.14	45.75±16.87	0.383
Baseline testosterone (ng/mL)	0.27±0.15	0.30±0.18	0.63±3.29	0.170
AMH (ng/mL), mean±SD	4.11±2.42	4.04±2.52	3.96±2.63	0.921
AFC (n), mean±SD	15.72±4.55	14.99±5.30	15.25±4.94	0.667
GnRH-a on day 1
LH (U/L), mean±SD	0.78±0.79	0.91±5.23	0.32±0.35	0.499
Estradiol (pg/mL), mean±SD	27.80±17.93	32.52±89.46	23.47±9.27	0.555
Progesterone (ng/mL), mean±SD	0.41±0.18	0.54±0.47^a*	0.46±0.28	0.025
Follicles (n), mean±SD	16.00±4.47	16.00±5.53	16.62±5.49	0.610
During ovarian stimulation
Preliminary GnRH-a dose (IU), mean±SD	168.48±43.44	192.98±52.75^a**	200.64±67.37^a**	<0.001
Maximum daily GnRH-a dose (IU), mean±SD	188.26±45.17	248.21±59.93^a**	278.87±65.16^ab**	<0.001
Duration of GnRH-a (days), mean±SD	10.12±2.00	11.43±2.55^a**	12.53±2.14^ab**	<0.001
Total GnRH-a dose (IU), mean±SD	1789±569	2447±1068^a**	2834±978^ab**	<0.001
Mature follicles ≥ 14 mm (n), mean±SD	10.88±3.91	11.07±4.44	10.38±4.23	0.431
Estradiol (pg/mL), mean±SD	2821±1256	2764±1314	2568±1426	0.389
LH (U/L), mean±SD	1.69±2.43	1.91±12.62	0.63±0.43	0.554
Progesterone (ng/mL), mean±SD	0.86±0.40	6.01±72.91	0.65±0.42	0.629
Endometrial thickness (mm), mean±SD	10.31±2.81	11.30±2.48^a*	11.14±2.67^a*	0.002
Retrieved oocytes, mean±SD	15.10±6.19	14.61±5.43	14.55±4.67	0.700
Mature oocytes, mean±SD	12.90±5.43	12.72±4.95	12.67±5.66	0.941
Fertilized oocytes, mean±SD	10.57±4.61	10.47±4.41	10.45±4.99	0.975
Fertilization rate (%), mean±SD	83.01±13.07	82.53±13.47	82.17±11.68	0.919
Total embryos, mean±SD	10.35±4.61	10.23±4.27	10.16±4.91	0.955
Total high-quality embryos, mean±SD	8.39±4.16	7.86±3.82	7.91±3.91	0.222
High-quality embryo rate (%), mean±SD	81.21±17.80	76.70±19.26	78.23±21.00	0.116
Blastocysts, mean±SD	5.55±2.98	4.84±2.85	4.73±3.24	0.069
Blastocyst formation rate (%)	54.22±21.02	48.13±22.26^a*	49.77±21.42	0.049
Fresh embryo transfer cycles, n	83	197	66
Pregnancy outcomes
Biochemical pregnancy, n (%)	68/83 (81.9)	149/197 (75.6)	53/66 (80.3)	0.426
Clinical pregnancy, n (%)	55/83 (66.3)	123/197 (62.4)	46/66 (69.7)	0.749
Live birth, n (%)	46/83 (55.4)	110/197 (55.8)	40/66 (60.6)	0.819
Cumulative live birth, n (%)	78/115 (67.8)	161/244 (66.0)	56/90 (62.2)	0.978

Notes: *P<0.05, **P<0.001. ^avs. Group I. ^bvs. Group II. Group I: FSH; group II: FSH+HMG/r-LH; group II: FSH+HMG+r-LH.

Abbreviations: SD, standard deviation; BMI, body mass index; FSH, follicle-stimulating hormone; HMG, human menopausal gonadotropin; r-LH, recombinant luteinizing hormone; AMH, anti-Mullerian hormone; AFC, antral follicle count; LH, luteinizing hormone.

Table 2 Characteristics of Women Aged ≥32 Years and Overweight

Variables	Age ≥32 Years				Overweight
	Group I (n=115)	Group II (n=244)	Group III (n=97)	P	Group I (n=115)	Group II (n=244)	Group III (n=97)	P
Age (years), mean±SD	34.25±1.93	34.61±2.12	35.28±2.57^a*	0.045	31.34±3.32	31.85±4.06	31.67±4.41	0.803
BMI (kg/m^2), mean±SD	22.09±2.94	21.76±3.12	23.39±3.40^ab*	0.006	25.67±2.15	25.43±2.03	25.93±1.85	0.293
Endometrial thickness (mm), mean±SD	9.70±2.93	11.10±2.55^a*	11.05±2.16^a*	0.009	10.82±3.29	11.36±2.39	11.79±3.00	0.190
Blastocysts, mean±SD	5.55±2.83	4.73±2.81	4.91±3.24	0.245	5.32±2.78	5.09±2.70	4.55±3.14	0.772
Blastocyst formation rate (%), mean±SD	55.68±20.62	47.99±22.81	45.17±20.61	0.058	55.39±21.18	52.25±23.96	50.16±24.70	0.659
Fresh embryo transfer cycles (n)	37	101	39		28	60	36
Biochemical pregnancy, n (%)	29/37 (78.4)	74/101 (73.3)	30/39 (76.9)	0.793	20/28 (71.4)	46/60 (76.7)	30/36 (83.3)	0.518
Clinical pregnancy, n (%)	22/37 (59.5)	61/101 (60.4)	26/39 (66.7)	0.757	15/28 (53.6)	31/60 (51.7)	26/36 (72.2)	0.122
Live birth, n (%)	17/37 (45.9)	53/101 (52.5)	21/39 (53.8)	0.748	11/28 (39.3)	28/60 (46.7)	24/36 (66.7)	0.063
Cumulative live birth, n (%)	29/51 (56.9)	72/123 (58.5)	31/54 (57.4)	0.976	24/38 (63.2)	54/79 (68.4)	34/51 (66.7)	0.976

Notes: *P<0.05, **P<0.001. ^avs. Group I. ^bvs. Group II. Group I: FSH; group II: FSH+HMG/r-LH; group II: FSH+HMG+r-LH.

Abbreviations: SD, standard deviation; BMI, body mass index.

Table 3 Direct Medical Costs for Oocyte Retrieval

Variables	Group I (n=115)	Group II (n=244)	Group III (n=97)	P
Oocyte retrieval cycle (CNY/per), mean±SD	14940±1983	15,524±1996^a*	17,052±2325^ab**	<0.001
Embryo (CNY/per), mean±SD	1826±1099	1860±1018	2264±1654^ab*	0.011
Blastocyst (CNY/per), mean±SD	3874±3314	4610±3685	5304±4467 ^a*	0.026

*P<0.05, **P<0.001. ^avs. Group I. ^bvs. Group II. Group I: FSH; group II: FSH+HMG/r-LH; group II: FSH+HMG+r-LH.

Abbreviation: SD, standard deviation.

Data Sharing Statement

The datasets used and/or analysed during the current study available from the corresponding author on reasonable request.