Detection of Rickettsia Endosymbiont Bemisia Tabaci in the Amputated Limbs of Three Buerger’s Disease Patients

Figures & data

Table 1 Primers and Program Used for PCR Amplification for 16srRNA Specific for Rickettsiales.

Primers	Program
“F:5ʹ-CGCAACCCTCATTCTTATTTGC-3ʹ” “R:5ʹ-CCTCTGTAAACACCATTGTAGCA-3ʹ”	DNA denaturation at 94 ◦C for 5 min, PCR amplification for 35 cycles as follows: denaturation at 94 ◦C for 60 s, annealing at 56 ◦C for 45 s and extension at 72◦C for 60 s.

Note: Data from Giulieri et al.⁶ 

Figure 1 Case 1, (A) gangrene of forefoot; (B) angiography characteristics for Buerger’s disease; (C) infiltration of inflammatory cells in all layers of the vessel and organized thrombus are suggestive for Buerger’s disease.

Figure 2 Case 2, (A) gangrene of forefoot; (B) angiography characteristics for Buerger’s disease; (C) organized thrombus in medium and small sized vessels along with intima thickening and infiltration of inflammatory cells in all layers of the vessels and are suggestive for Buerger’s disease.

Figure 3 Case 3, (A) gangrene of forefoot, (B) angiography characteristics for Buerger’s disease; (C) Infiltration of inflammatory cells in all layers of the vessel and organized thrombus are suggestive for Buerger’s disease.

Table 2 Primers and Program Used for PCR Amplification for 16srRNA Specific for Rickettsia Endosymbiont Besimia Tabaci

Primers	Program
F:5ʹ- CATCATGGCCCTTACGGGTT-3ʹ R:5ʹ- GTCGCTAATTTTACCGTGGTTGG-3ʹ	DNA denaturation at 94 ◦C for 5 min, PCR amplification for 40 cycles as follows: denaturation at 94 ◦C for 60 s, annealing at 53 ◦C for 45 s and extension at 72◦C for 60 s.

Figure 4 Alignment of the PCR product sequence of 16srRNA of Rickettsia Endosymbiont Besimia Tabaci with submitted sequences for the same pathogen in NCBI.

Giulieri S, Jaton K, Cometta A, Trellu LT, Greub G. Development of a duplex real-time PCR for the detection of Rickettsia spp. and typhus group rickettsia in clinical samples. FEMS Immunol Med Microbiol. 2012;64(1):92–97. doi:10.1111/j.1574-695X.2011.00910.x

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