The Precision Allergy Molecular Diagnosis (PAMD@) in Monitoring the Atopic March in a Child with a Primary Food Allergy: Case Report

Figures & data

Table 1 Study of Specific IgE in 29-Month Lasting Follow-Up

Allergen Component		Euroline Pediatric Profile*	Polycheck Egg Components **	FABER***	ALEX****	ALEX****
Unit of measure		kU/L	kU/L	FIU/mL	kU/L	kU/L
Date of execution		09/2019	10/2019	11/2019	02/2021	01/2022
Time since introducing an elimination diet		Without an elimination diet		After 2 months	After 17 months	After 28 months
Age of the patient in months		9	10	11	26	37
Food allergens	Alpha-lactalbumin (Bos d 4)	1.70		0.54 (n)	≤0.1 (n)	≤0.10 (n)
	Beta-lactoglobulin (Bos d 5)	36.00		0.81 (n)	0.32 (n)	1.10 (n)
	Casein (Bos d 8)	16.80		1.62 (n)	1.15 (n)	4.52 (n)
	Ovomucoid (Gal d 1)		3.10 (n)	4.29 (n)	2.74 (n)	2.39 (n)
	Ovalbumin (Gal d 2)		3.50 (n)	<0.01 (n)	3.66 (n)	0.79 (n)
	Ovotransferrin (Gal d 3)		5.80 (n)	0.81 (n)	0.66 (n)	0.43 (n)
	Lysozyme (Gal d 4)		23.00 (n)	<0.01 (n)	≤0.10 (n)	≤0.10 (n)
Inhalation allergens	Birch (Bet v 1)			<0.01 (r)	0.27 (r)	2.33 (r)
	Timothy (Phl p 1)			<0.01 (r)	≤0.10 (r)	1.51 (r)
	Alternaria alternata (Alt a 1)			<0.01 (r)	≤0.10 (r)	5.35 (r)

Notes: (r) – recombinant (n) – natural; filling colour grey – no data, due to non-performance of the measurement at a given time or no such measurement on a given test. *The results were obtained with the use of a multi-parameter Euroline immunoassay (Euroimmun, PerkinElmer Germany Diagnostics GmbH containing 27 allergens). Positive result ≥ 0.35 kU/L. **The results were obtained with the use of multi-parameter Polycheck assay (Biocheck GmBH, Germany). Positive result ≥0.35 kU/L. ***Multi-parameter FABER immunoassay (CAAM, Rome, Italy) - negative result <0.01 FIU/mL, borderline >0.01/<0.30 FIU/mL, positive ≥0.30 FIU/mL. ****Multi-parameter ALEX immunoassay (MacroArray Diagnostics, Vienna, Austria) – negative or borderline result <0.3 kU/L, positive result ≥0.3 kU/L. Bold numbers – positive result (result ≥0.35 kU/L). Underlined numbers – borderline result (result between 0.1 kU/L and <0.35 kU/L).

Table 2 Assessment of Asthma Predictive Index in the Presented Patient

Assessed Criteria*	Patient’s Signs and Symptoms
Major Criteria
Asthma in parents	Yes
Atopic eczema	Yes
Minor Criteria
Allergic rhinitis	Yes
Wheezing without infection	Yes
Peripheral eosinophilia >4%	Yes

Notes: *The criteria for the diagnosis of asthma according to Rodríguez JA, Holberg CJ, Wright AL and Martinez FD. showed in article A clinical index to define risk of asthma in young children with recurrent wheezing. Am J Respir Crit Care Med. 2000; 162: 1403–1406.³

Castro-Rodríguez JA, Holberg CJ, Wright AL, Martinez FD. A clinical index to define risk of asthma in young children with recurrent wheezing. Am J Respir Crit Care Med. 2000;162(4):1403–1406. doi:10.1164/ajrccm.162.4.9912111

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