Figures & data
Table 1 Donation History, Results of Laboratory Analyses, and Percentage Distribution of Responses to Questionnaire Items by Gender and Age Group (n = 86)
Figure 1 Representative flow cytometry plots of activated platelets in pre-donation whole blood samples. (A) Whole blood samples were incubated with anti-CD61 and anti-CD62P (left) or isotype control (middle), washed and analyzed by flow cytometry. Histogram overlay of CD61-positive events is shown to the right. (B) Flow cytometry dot plots showing whole blood samples stimulated with ADP.
![Figure 1 Representative flow cytometry plots of activated platelets in pre-donation whole blood samples. (A) Whole blood samples were incubated with anti-CD61 and anti-CD62P (left) or isotype control (middle), washed and analyzed by flow cytometry. Histogram overlay of CD61-positive events is shown to the right. (B) Flow cytometry dot plots showing whole blood samples stimulated with ADP.](/cms/asset/46b64804-d507-4d1a-a425-33bf9cc9f0d7/djbm_a_12167240_f0001_b.jpg)
Table 2 Correlation of Platelet Activation in Pre-Donation Blood Samples with Collection Time and Other Covariates (n = 84)
Table 3 Results of Univariate (Unadjusted) and Backward Multivariable Linear (Adjusted) Models for Association of Platelet Activation in Whole Blood (n = 84) to Collection Time and Other Factors
Figure 2 Relationship between (A) the percentage of P-selectin-positive platelets and (B) mean platelet volume (MPV, a surrogate marker of platelet activation) with the time of day at which blood samples were collected (collection time). The percentage of P-selectin-positive platelets, as assessed by flow cytometry, correlated inversely (r = –0.35), with the collection time. MPV, as measured via a Sysmex XE-5000 analyzer, was also inversely correlated with the collection time, although it did not reach statistical significance in this study.
![Figure 2 Relationship between (A) the percentage of P-selectin-positive platelets and (B) mean platelet volume (MPV, a surrogate marker of platelet activation) with the time of day at which blood samples were collected (collection time). The percentage of P-selectin-positive platelets, as assessed by flow cytometry, correlated inversely (r = –0.35), with the collection time. MPV, as measured via a Sysmex XE-5000 analyzer, was also inversely correlated with the collection time, although it did not reach statistical significance in this study.](/cms/asset/0d588ab7-caf0-4496-9f59-4c7c1179d158/djbm_a_12167240_f0002_b.jpg)