Circulating hematopoietic progenitors and CD34⁺ cells predicted successful hematopoietic stem cell harvest in myeloma and lymphoma patients: experiences from a single institution

Figures & data

Table 1 Clinical characteristics of patients in relation with HSC collection

	Minimally required HSC yield			Optimal HSC yield
	No (n=12)	Yes (n=38)	P-value	No (n=32)	Yes (n=18)	P-value
Age (years)
<60	6	27	0.294*	20	13	0.700*
≥60	6	11		12	5
Sex
Male	7	19	0.863*	15	11	0.501*
Female	5	19		17	7
Diagnosis
Lymphoma	5	20	0.741*	13	12	0.141*
Myeloma	7	18		19	6
Disease status
CR	3	6	0.409*	8	1	0.125*
Non-CR	8	31		22	17
Heavy treatmenta
Yes	3	11	1.000*	8	6	0.763*
No	9	27		24	12
Prior radiotherapy
Yes	3	10	1.000*	8	5	1.000*
No	9	28		24	13
Mobilization regimen
C/T + G-CSF	6	27	0.294*	16	17	0.004*
G-CSF only	6	11		16	1
Total apheresis days (mean ± SEM)	2.50±0.26	2.05±0.13	0.077**	2.38±0.15	1.78±0.13	0.013**

Notes:

a Received more than two lines of chemotherapy.

* P-value for Chi-square test.

** P-value for Mann–Whitney U test.

Abbreviations: HSC, hematopoietic stem cell; CR, complete remission; C/T, systemic chemotherapy; G-CSF, granulocyte colony-stimulating factor; SEM, standard error of the mean.

Table 2 Laboratory variables for the prediction of minimally required or optimal HSC collection

	Minimally required HSC yield			Optimal HSC yield
	No (n=12)	Yes (n=38)	P-value*	No (n=32)	Yes (n=18)	P-value*
HPC
Absolute count	14.49±4.89	86.87±14.68	0.000	38.09±6.50	125.33±27.08	0.011
Slope	3.87±0.78	22.40±12.48	0.686	9.15±4.85	38.77±29.55	0.269
Ratio	4.33±0.88	23.41±12.48	0.384	9.78±4.68	39.77±29.55	0.210
CD34^+ cells
Absolute count	5.33±2.40	54.30±10.37	0.000	24.53±5.23	75.76±20.06	0.006
MNC
Absolute count	3,045±548	4,126±651	0.586	4,655±714	2,466±528	0.028
Slope	0.89±0.53	1.17±0.20	0.138	1.05±0.24	1.20±0.36	0.845
Ratio	1.89±0.53	2.17±0.20	0.138	2.05±0.24	2.20±0.36	0.845

Notes: Data are presented as mean ± standard error.

* P-value for Mann–Whitney U test.

Abbreviations: HSC, hematopoietic stem cell; HPC, hematopoietic progenitor cells; MNC, mononuclear cells.

Table 3 Cutoff values for HPCs and CD34⁺ cells that are predictive of minimally required or optimal HSC yield

	Minimally required HSC yield			Optimal HSC yield
	No (n=12)	Yes (n=38)	P-value*	No (n=32)	Yes (n=18)	P-value*
HPC (1/mm^3)			0.001			0.034
<20	9	8		14	3
20–60	3	11		10	4
>60	0	19		8	11
CD34^+ cell (1/mm^3)			0.000			0.024
<10	10	7		14	3
10–35	2	11		10	3
>35	0	19		8	11

Note:

* P-value for Chi-square test.

Abbreviations: HPCs, hematopoietic progenitor cells; HSC, hematopoietic stem cell.