Curcumin Supplementation Alleviates Polymyxin E-Induced Nephrotoxicity

Figures & data

Table 1 Plasma Biochemical Measurements in Polymyxin E (PolyE)-Treated Mice

Plasma Marker	Control	PolyE 15 mg/kg	PolyE + CUR 10 mg/kg	PolyE + CUR 100 mg/kg	PolyE + CUR 200 mg/kg
BUN (mg/dl)	44 ±8	85 ±13*	53±9^a	54 ±4^a	54 ±6^a
Creatinine (mg/dl)	0.48±0.12	0.83±0.15*	0.56±0.06^a	0.55±0.05^a	0.51±0.05^a

Notes: Data are given as mean ± SD (n = 8). *Indicates significantly different as compared with the control group (P < 0.01). ^aIndicates significantly different as compared with PolyE 15 mg/kg group (P < 0.05).

Abbreviation: CUR, curcumin.

Figure 1 Kidney tissue markers of oxidative stress in polymyxin E (PolyE)-treated mice and the effect of curcumin (CUR) treatment. Data are given as mean ± SD (n = 8). ***Indicates significantly different as compared with the control group (P < 0.01). ^aIndicates significantly different as compared with the PolyE group (P < 0.05).

Table 2 Grade of Kidney Tissue Histopathological Alterations in Polymyxin E (PolyE)-Treated Mice

Treatments	Interstitial Nephritis	Tissue Necrosis	Glomerular Atrophy
Control	0 (0, 0)^#	0 (0, 0)^#	0 (0, 0)^#
PolyE 15 mg/kg	2 (2, 2)	4 (3, 4)	3 (3, 3)
PolyE + CURC 10 mg/kg	1 (0, 1)	2 (1, 1)^#	2 (1, 1)^#
PolyE + CURC 100 mg/kg	0 (0, 0)^#	1 (0, 1)^#	2 (1, 1)^#
PolyE + CURC 200 mg/kg	0 (0, 0)^#	1 (0, 1)^#	1 (0, 1)^#

Notes: Data are represented as median and quartiles for eight random pictures per group. ^#Indicates significantly different as compared with the PolyE-treated group (P < 0.01).

Abbreviation: CUR, curcumin.

Figure 2 Kidney histopathological alterations in polymyxin E (PolyE)-treated animals. Typical kidney tissue histopathology (A). Tubular necrosis, interstitial inflammation, and atrophy were evident in PolyE-treated (15 mg/kg/day, i.v, seven consecutive days) animals (B). It was found that curcumin administration significantly decreased PolyE-induced renal injury (PolyE + curcumin; (C–E) for 10, 100, and 200 mg/kg of curcumin, respectively). The grades of kidney histopathological alterations are given in Table 2.