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Original Research

In vitro Antioxidant and in vivo Wound Healing Activities of the 80% Methanol Extract and Solvent Fractions of Seeds of Brassica carinata A. Braun (Brassicaceae) in Mice

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Pages 463-474 | Published online: 06 Nov 2020

Figures & data

Figure 1 Wound created at day 0.

Figure 1 Wound created at day 0.

Table 1 Preliminary Phytochemical Analysis of 80% Methanol Extract and Solvent Fractions of B. carinata Seeds

Table 2 Effect of Topical Application of the 80% Methanolic Crude Extracts Ointments of the Seeds of B. carinata on Wound Contraction of Excision Wound Model in Mice

Figure 2 Progress on percentage of wound area contraction of negative and positive controls, and 5% w/w and 10% w/w crude extract ointments across post-wounding days in mice in excision model.

Figure 2 Progress on percentage of wound area contraction of negative and positive controls, and 5% w/w and 10% w/w crude extract ointments across post-wounding days in mice in excision model.

Figure 3 Percentage of wound area contraction effects of crude extract ointments of seeds of B. carinata in mice in excision model.

Abbreviations: SO, simple ointment; NF, 0.2% nitrofurazone; CE, crude extract.
Figure 3 Percentage of wound area contraction effects of crude extract ointments of seeds of B. carinata in mice in excision model.

Figure 4 Epithelialization periods of B. carinata seeds crude extract and solvent fractions treated groups in excision model. Values are expressed as mean±SEM and analyzed by one-way ANOVA followed by Tuckey post-hoc test (n=6 animals in each group).

Note: **P<0.01, ***P<0.001.
Abbreviations: SO, simple ointment; NF, 0.2% nitrofurazone; CE, crude extract; CF, chloroform fraction; EAF, ethyl acetate fraction; AQF, aqueous fraction.
Figure 4 Epithelialization periods of B. carinata seeds crude extract and solvent fractions treated groups in excision model. Values are expressed as mean±SEM and analyzed by one-way ANOVA followed by Tuckey post-hoc test (n=6 animals in each group).

Table 3 Effects of Topical Application of the Chloroform, Ethyl Acetate, and Aqueous Fractions of the Seeds of B. carinata on Wound Contraction of Excision Wound Model in Mice

Figure 5 Percentage of wound area contraction effects of chloroform, ethyl acetate, and aqueous fractions of B. carinata seed extract in excision model.

Abbreviations: SO, simple ointment; NF, 0.2% nitrofurazone; CE, crude extract; CF, chloroform fraction; EAF, ethyl acetate fraction; AQF, aqueous fraction.
Figure 5 Percentage of wound area contraction effects of chloroform, ethyl acetate, and aqueous fractions of B. carinata seed extract in excision model.

Table 4 Effects of Topical Application of the 80% Methanolic Crude Extracts Ointment of B. carinata Seeds on Tensile Strength of Incision Wound Model in Mice

Table 5 Anti-Oxidant Activities of the Crude Extract and Solvent Fractions of Seeds of B.carinata in DPPH Assay Model