Evaluation of Analgesic and Anti-inflammatory Activities of Methanolic Leaf and Root Extracts of Gomphocarpus purpurascens A. Rich (Asclepiadaceae) in Mice

Figures & data

Table 1 Preliminary Phytochemical Screening of 80% Methanol Extract of the Leaf and Roots of G. Purpurasence

Secondary Metabolites	Root Extract	Leaf Extract
Saponin	+	+
Alkaloid	+	+
Tannin	+	+
Phenol	+	+
Flavonoid	_	+
Anthraquinone	+	+
Triterpenoid	+	+
Steroid	+	+

Table 2 Effect of 80% Methanolic Root Extracts of G. Purpurasence on Acetic Acid-Induced Writhing in Mice

Group	Number of Writhing(mean)	% of Inhibition
DW	29.5 ±0.76	---
Asprin150 mg (10mL/kg)	4.5 ±0.56^aAbAcB	84.7
GP (100mg/kg)	24.6 ±1.86^aB	16.6
GP (200mg/kg)	9.17 ±2.48^aAbA	68.9
GP (400mg/kg)	5 ±0.89 ^aAbAcB	83

Notes: Values are expressed as Mean ± S.E.M (n=6); analysis was performed with One-Way ANOVA followed by Tukey post hoc test; ^aAgainst the control, ^bagainst GP100, ^cagainst GP200, ^AP<0.001 ^BP<0.01.

Table 3 Effect of 80% Methanolic Root Extracts of G. Purpurasence on Hot Plate Method

Group	Latency (Sec) ± SEM
	0 Min	30Min	60Min	90Min	120Min
DW	7.583±0.36	3.880±0.236	7.983±0.28	5.486±0.279	5.395±0.405
MO (10mg/kg)	6.666±0.538	6.905±1.059	8.805±0.168^aB	10.581±0.940^aB	9.186±0.886
GP100	6.710±0.563	7.258±0.236	6.203±0.40	6.680±0.392	8.245±0.679
GP200	6.540±0.202	8.443±0.369	7.901±0.269	8.1057±0.488	9.781±0.857^aA
GP400	6.870±0.079	6.593±0.673	8.706±0.449	10.583±0.500	9.986±0.855^aA

Notes: Values are expressed as Mean ± S.E.M (n=6); analysis was performed with One-Way ANOVA followed by Tukey post hoc test; ^aAgainst the control, ^AP<0.01, ^BP<0.001.

Table 4 Effect of 80% Methanolic Extract of Leaf of G. Purpurasence on Carrageenan-Induced Paw Edema in Mice

Treatment Mg/kg	Edema Volume (mL)/ Percent Edema Inhibition
	Baseline	1 Hr	2 Hr	3 Hr	4 Hr
2% TW 80	0.0833±0.0196	0.1483±0.0104	0.1617±0.00981	0.1583±0.00983	0.1550±0.0054
Indomethacin 25 mg	0.11±0.00803	0.1500±0.0081/38.5	0.1167±0.0040**/91.4	0.1167±0.0055***/91.1	0.1117±0.0042**/97.6
100mg	0.0933±0.0033	0.1183±0.0172*/61.5	0.1083±0.0047***/80.8	0.1000±0.0036***/91	0.0967±0.0049***/95.2
200mg	0.0883±0.0065	0.1233±0.0136*/46	0.1117±0.0079***/70	0.1013±0.0044***/82.6	0.0950±0.0034***/90.6
400mg	0.1017±0.013	0.1183±0.0075*/82	0.1083±0.006577y 54***/97.8	0.0967±0.0042***/93	0.1067±0.01116***/93

Notes: Values are expressed as Mean ± S.E.M (n=6); analysis was performed with One-Way ANOVA followed by Tukey post hoc test;*P<0.05, **P<0.01, ***P<0.001 against negative control.

Table 5 Effect of 80% Methanolic Extract of Leaf of G. Purpurasence on Formalin Induced Paw Edema

Treatment Group	Mice Paw Volume in Measured on Different Days
	Day1	Day2	Day3	Day4	Day5	Day6	Day7
-ve	0.1550±.0225	0.1583±0.0248	0.1550±.0234	0.1683±0.02483	0.1567±.0206	0.1633±0.0088	0.1500±.0057
+ve	0.1550±.0225	0.1620±.0288	0.1567±.0163	0.1417±.0189	0.1167±0.018***	0.1150±.0016***	0.1033±0.0034***
100mg	0.1833±.0344	0.1650±0.0258	0.1867±0.0273	0.1583±.0147	0.1150±.0147***	0.1033±.0049***	0.1000±.0068***
200mg	0.1617±.0256	0.1600±.0194	0.1783±0.0248	0.1400±.0219	0.1233±.0219**	0.1000±.0044***	0.100±.00683***
400mg	0.1850±0.0441	0.1417±.0147	0.1800±0.0400	0.1350±.0151*	0.1010±.0151***	0.1200±.0077**	0.1167±.00615*

Notes: Values are expressed as Mean ± S.E.M (n=6); analysis was performed with One-Way ANOVA followed by Tukey post hoc test; *P<0.05, **P<0.01, ***P<0.001 against negative control.