Pharmacological Evaluation of the Anesthetic and Analgesic Potential of Injection Harsha 22: A Novel Polyherbal Local Anesthetic Formulation Intended for Parenteral Administration in Wistar Albino Rats

Figures & data

Figure 1 The four anatomical sites selected for administering the Injection Harsha 22.

Table 1 Sterility Test Result of Injection Harsha 22

Media Tested	Presence or Absence of Microbes
Fluid thioglycolate	Absent
Soya bean casein digest medium	Absent

Figure 2 Tail flick latencies of rats after treatment with Injection Harsha 22 at different time intervals. Here Control: Normal saline, Test- Injection Harsha 22 and Standard- Lignocaine.

Figure 3 (A) Maximum possible effects - MPE (%) and (B) Area under the curve (AUC) of treatment groups at different time intervals. Here Control: Normal saline, Test- Injection Harsha 22 and Standard- Lignocaine.

Table 2 Ranking of Analgesia at Different Anatomical Sites Anaesthetized with Injection Harsha 22. Data are of 3 Replicates ± SEM; 0–25 = No Analgesia; 25–50 = Low Analgesia; 50–75 = Moderate Analgesia; 75–100 = High Analgesia

Groups	Tail	Left Paralumbar	Right Paralumbar	Linea Alba
Group 1- Control	25±00	25±00	25±00	25±00
Group 2- Test	100±00	91.67±14.43	100±00	75±00
Group 3- Standard	75±00	58.33±14.43	50±00	66.67±14.43

Figure 4 Percentage (%) of animals with analgesia after treatment. Here Control: Normal saline, Test- Injection Harsha 22 and Standard- Lignocaine.

Table 3 Effect of Injection Harsha 22 on the Hematological Parameters in Experimental Groups

Groups	WBC (10^3 Cells/µL)	Lymphocytes (10^3 Cells/µL)	Granulocytes (10^3 Cells/µL)	Hemoglobin (g/dL)	RBC (10^3 Cells/µL)	Hematocrit (%)	Platelet Count (10^3 Cells/µL)
Control	7.6±0.344	5.416±0.215	0.616±0.174	15.2±0.308	8.055±0.180	39.15±0.750	674.33±31.22
Test	7.333±0.563	4.833±0.427	0.95±0.232	14.916±0.110	7.533±0.315	39.683±1.028	670±30.337
Standard	8.016±0.443	5.783±0.510	1.2±0.409	15.1±0.286	7.876±0.193	39.233±1.082	710.16±44.944

Note: Values are expressed as mean ± SEM.

Table 4 Effect of Injection Harsha 22 on the Serum Lipid Profile

Groups	Total Cholesterol (mg/dL)	Triglycerides (mg/dL)	HDL (mg/dL)	VLDL (mg/dL)	LDL (mg/dL)
Control	86.733±6.905	77.7±9.786	11.816±4.621	15.54±1.957	59.37±5.684
Test	100.783±8.759	69.7±9.723	24.45±4.952	13.94±1.944	100.783±8.759
Standard	102.3±7.772	69.733±9.712	27.866±2.230	13.946±1.942	60.486±6.903836

Note: Values are expressed as mean ± SEM.

Table 5 Effect of Injection Harsha 22 on the Serological Parameters in Experimental Groups

Groups	AST (U/L)	ALT (U/L)	ALP (U/L)	Total Protein (g/dL)	Albumin (g/dL)	Bilirubin Total (mg/dL)	Urea (mg/dL)	Creatinine (mg/dL)	Uric Acid (mg/dL)
Control	125.16±6.439	35.31±2.37	243.16±23.76	7.273±0.336	2.066±0.066	0.066±0.021	38.55±4.931	0.483±0.044	0.736±0.091
Test	119.45±6.047	42.18±0.69**	223.91±13.724	6.736±0.128	1.9±0.036	0.15±0.034	42.566±2.33	0.491±0.030	0.703±0.041
Standard	122.2±6.327	41.95±0.676**	236.88±17.949	7.063±0.183	1.933±0.049	0.15±0.034	40.06±2.277	0.52±0.019	0.623±0.095

Notes: Values are expressed as mean±SEM. **p < 0.01 when compared with control Group.