ELR positive CXCL chemokines are highly expressed in an animal model of ulcerative colitis

Figures & data

Table 1 Primers used in this study

Gene	Forward primer	Reverse primer
Gapdh	AGTTCAACGGCACAGTCAAG	ATACTCAGCACCAGCATCAC
CXCL1	AGACAGTGGCAGGGATTCAC	AAGCCTCGCGACCATTCTTG
CXCL2	TCCTCAATGCTGTACTGGTC	TGAAGTCAACCCTTGGTAGG
CXCL3	TCAATGCTGCACTGCTTCTG	TCCTTGAGAGTGGCTATGAC
CXCL4	TTCTTCTGGGTCTGCTGTTG	TGCGTTTGAGATGGATCCTG
CXCL6	TTTCTGCTGCTGTTCACACTG	TAGCTATGACTTCCACCTTGG
CXCL7	TTCAGACTCAGACCTACATC	AATGGCTCGTTGTTATCAGG
CXCL9	TGCCTAGACCCAGATTCAGC	AGATGCAGAGCGCTTGTTGG
CXCL10	TCCTGCAAGTCTATCCTGTC	TGGCTTCTCTCTAGTTACGG
CXCL11	AGATTGCAGAACTCGCTAGG	AGACGATAACTCGGAGTGTG
CXCL12	ATCCTCAACACTCCAAACTG	TTAAGGCTTTGTCCAGGTAC
CXCL13	TTCTTCTACTGGCCATCTGC	TTCCCAGGGCGTATAACTTG
CXCL14	TTCGGTGCATATGCAAACAGC	AGCACTCATGAATCTCAGCAC
CXCL16	TGTTGGGATTAAAGGACTGC	AACCTCGAACTTTACACTCC
CXCL17	TCTTGCTCTCGTCTTCTCATG	AGAGGCTAGAAGCTTCATTGC

Figure 1 Macroscopic examinations of colon tissue in the UC and control groups. (A) Gross macroscopic structure of colon in control and UC Groups. (B) Weight to length ratio of the last 6 cm of colon tissue. The induction of colitis model was performed once. Each column represents fold change±SEM for six male Wistar rats. * P≤0.05.

Abbreviation: UC, ulcerative colitis.

Figure 2 Histopathological findings of UC and control groups. The experiments were performed using optical microscopy with 10X and 40X magnification. (A&B) Normal colon, (C&D) UC.

Abbreviation: UC, ulcerative colitis.

Figure 3 ELR⁺ CXC chemokines are highly upregulated in UC. Each column represents a fold change ± SEM for six male Wistar rats. Fold change calculated using this formula: 2^−ΔCT[2 ^{− (CT CXCL – CT GAPDH)}] UC group/2^−ΔCT[2 ^{− (CT CXCL – CT GAPDH)}] control. The statistical results in the UC group show a significant difference compared to the control. * P≤0.05, ** P≤0.01, *** P<0.0001.

Abbreviation: UC, ulcerative colitis.

Figure 4 Expression comparison of ELR⁻ CXC chemokines in AA-induced UC. Each column represents fold change ± SEM for six male Wistar rats. Fold change calculated using this formula: 2^−ΔCT[2 ^{− (CT CXCL – CT GAPDH)}] UC group/2^−ΔCT[2 ^{− (CT CXCL – CT GAPDH)}] control. The statistical results of CXCL4, CXCL13, CXCL14, and CXCL16 chemokines in UC group had a significant difference with the control group (P≤0.05). The statistical results of CXCL9, CXCL10, CXCL11, CXCL12 and CXCL17 chemokines in UC group showed no significant difference with control group (P>0.05). ** P≤0.01, *** P<0.0001.

Abbreviation: UC, ulcerative colitis.