Overexpression of human SOD1 improves survival of mice susceptible to endotoxic shock

Figures & data

Figure 1 Cu-Zn superoxide dismutase (SOD1) activity in heart tissues of two SOD1 transgenic (tg) strains and their littermates.

Notes: SOD1 activity was determined using a polyacrylamide activity gel as described in Materials and methods. Upper panel is the image of the activity gel. Each lane in the gels represents an individual mouse. The intensities of major bands of each mouse were quantified and summarized in the dot plots. The horizontal lines within the dot plots represent the average intensities of the respective strains of tg mice and their wild-type (WT) littermates. The asterisks indicate statistical significance P < 0.05 between transgenic mice and their WT littermates.

Figure 2 Cu-Zn superoxide dismutase (SOD1) activity in liver tissues of two SOD1 transgenic (tg) strains and their littermates.

Notes: SOD1 activities in livers were determined as described in Figure 1. The asterisks indicate statistical significance of P < 0.05 between tg mice and their wild-type (WT) littermates.

Figure 3 Cu-Zn superoxide dismutase (SOD1) activity in intestinal tissues of two SOD1 transgenic (tg) strains and their littermates.

Notes: SOD1 activities in intestines were determined as described in Figure 1. The asterisks indicate statistical significance P < 0.05 between tg mice and their wild-type (WT) littermates.

Figure 4 Survival analyses of two Cu-Zn superoxide dismutase (SOD1) transgenic (tg) strains after endotoxic shock. SOD1 tg and wild-type (WT) mice were subjected to intraperitoneal injection of LPS. (A) Survival curves for tg-JAX mice (solid line) and their WT littermates (dotted line). The diamond represents censored data. (B) Survival curves for tg-TX mice (solid line) and their WT litermates (dotted line).

Notes: The circle and diamond represent censored data. The asterisks indicate statistically significant differences in survival curves (P < 0.05).