Effect of PD-1 Inhibitor Combined with X-Ray Irradiation on the Inflammatory Microenvironment and Lung Tissue Injury in Mice

Figures & data

Figure 1 HE staining of lung tissue 1 month after irradiation and/or anti-PD-1 treatment in each group.

Notes: (A) Control; (B) PD-1 inhibitor alone; (C) Irradiation alone; (D) PD-1 inhibitor + irradiation.

Figure 2 Lung injury scores in the four groups of mice. (A) Inflammatory infiltrate score, (B) Alveoli deformation score. *P<0.05, **P<0.01, ***P<0.001, ns>0.05.

Abbreviation: RT, radiation therapy.

Figure 3 Masson staining of lung tissue 1 month after irradiation and/or anti-PD-1 treatment in each group.

Notes: (A) Control; (B) PD-1 inhibitor alone; (C) Irradiation alone; (D) PD-1 inhibitor + irradiation. Black Arrow: alveolar fibrosis.

Figure 4 Collagen volume fraction (%) in lung tissue in the four groups of mice. *P<0.05, ***P<0.001. Abbreviation: RT, radiation therapy.

Figure 5 Hydroxyproline content (µg/mg) in lung tissue in the four groups of mice. **P<0.01, ***P<0.001. Abbreviation:RT, radiation therapy.

Table 1 Populations of CD3+, CD4+ and CD8+ T-Lymphocytes in Each Group of Mice (%, Mean±SD)

Groups	CD3+	CD4+	CD8+
Control	32.20±3.58	17.70±2.98	14.40±2.63
PD-1 inhibitor alone	40.10±1.91	17.80±1.32	21.10±2.23
Irradiation alone	44.20±3.36	17.70±1.34	22.50±2.27
PD-1 inhibitor + irradiation	48.00±3.97	15.90±1.37	28.20±3.39

Figure 6 Immunohistochemistry of lymphocyte infiltration in lung tissue 1 month after irradiation and/or anti-PD-1 treatment in each group. (A) Control; (B) PD-1 inhibitor alone; (C) irradiation alone; (D) PD-1 inhibitor + irradiation.