Figures & data
Figure 1 Sequencing results from healthy controls. (A) The 389-base pair PCR amplification fragments with allele-specific primer set TM-Ala and TM-Val from controls analyzed by allele-specific amplification. Lanes show the band patterns in controls with the allele combinations C/T, T/T, and C/C at position 1418. M represents the DNA marker. (B–D) PCR amplification fragment direct and reverse sequencing map with three genotype (C/T, T/T, and C/C analyzed by allele-specific amplification) DNA as template, respectively. Because of reverse sequencing, A and G (indicated by arrow) should be T and C at position 1418. (E and F) Two kinds of TM-pGEM® plasmid clone reverse sequencing map confirm the T/C heterozygous genotype analyzed by allele-specific amplification. A and G indicated by arrow should also be T and C as above.
Table 1 TM455 genotype frequency and distribution of each genotype among ethnic subgroups (according to reports other than for the Chinese Han population)
Table 2 Single nucleotide polymorphism frequency in Hardy-Weinberg equilibrium among controls
Table 3 Allelic frequencies of Ala455 (A) and Val455 (V) in 384 healthy volunteers and in 144 patients with carotid atherosclerosis
Table 4 Inflammatory cytokines in 384 healthy control and in 144 patients with carotid atherosclerosis
Table 5 Inflammatory cytokines in 65 individuals with phenotype Ala/Ala (including cases and controls) and 183 individuals with phenotype Val/Val (including cases and controls)