Diagnostic and Prognostic Value of Dysregulated miR-10a-3p in Patients with Severe Pneumonia

Figures & data

Table 1 Clinical Data of the Study Population

Items	All Subjects (n = 145)		P
	Healthy Individuals (n = 75)	Severe Pneumonia (n = 70)
Gender (male/female)	47/28	45/25	0.342
Age (years)	56.7±13.8	57.2±13.2	0.207
BMI (kg/m^2)	22.74±1.68	22.56±1.91	0.519
WBC (10^9/L)	6.25±2.21	10.67±3.52	<0.001
NEU (10^9/L)	4.15±1.36	8.02±2.39	<0.001
LYM (10^9/L)	1.85±0.24	0.76±0.17	<0.001
CRP (mg/L)	3.33±1.42	43.73±8.68	<0.001
PCT (ng/mL)	0.24±0.08	3.08±1.27	<0.001
BNP (pg/mL)	105.36±20.28	329.57±49.79	<0.001
FIB (g/L)	2.58±0.39	3.78±1.13	<0.001
D-D (mg/L)	0.23±0.05	4.59±1.04	<0.001
APTT (s)	26.11±2.89	41.53±7.36	<0.001
PT (s)	11.57±2.36	14.23±4.73	0.001
Pathogenic bacteria (n, %)
Fungus	/	13 (18.57%)	/
Gram-positive bacteria	/	9 (12.86%)	/
Gram-negative bacteria	/	48 (68.57%)	/

Note: Data are expressed as n or mean ± standard deviation.

Abbreviations: BMI, body mass index; WBC, white blood cell count; NEU, neutrophil count; LYM, lymphocyte count; CRP, C-reactive protein; PCT, procalcitonin; BNP, brain natriuretic peptide; FIB, fibrinogen; D-D, D-dimer; APTT, activated partial thromboplastin time; PT, prothrombin time.

Table 2 Correlation Between miR-10a-3p and Clinical Characteristics

Characteristics	Correlation with miR-10a-3p (r)
WBC (10^9/L)	0.733***
NEU (10^9/L)	0.601***
LYM (10^9/L)	−0.665***
CRP (mg/L)	0.803***
PCT (ng/mL)	0.667***
BNP (pg/mL)	0.395**
FIB (g/L)	0.579***
D-D (mg/L)	0.498***
APTT (s)	0.404**
PT (s)	0.321**

Notes: **P < 0.01, ***P < 0.001.

Abbreviations: WBC, white blood cell count; NEU, neutrophil count; LYM, lymphocyte count; CRP, C-reactive protein; PCT, procalcitonin; BNP, brain natriuretic peptide; FIB, fibrinogen; D-D, D-dimer; APTT, activated partial thromboplastin time; PT, prothrombin time.

Figure 1 Relative expression level of serum miR-10a-3p. RT-qPCR results showed that the expression level of miR-10a-3p in severe pneumonia group was significantly enhanced compared with healthy individuals. ***P < 0.001.

Figure 2 ROC analysis. ROC curve had the ability to distinguish severe pneumonia from healthy people. The AUC of this curve is 0.881, the sensitivity and specificity are 75.7% and 84.0% respectively at the cut-off value of 1.274.

Table 3 Multivariate Cox Analysis of Clinical Characteristics Concerning Overall Survival

Characteristics	Multivariate Analysis
	HR	95% CI	P
MiR-10a-3p	3.793	1.084–13.272	0.037
Age	2.664	0.797–8.900	0.111
Gender	2.753	0.755–10.044	0.125
BMI	2.731	0.817–9.133	0.103
WBC	1.655	0.476–5.756	0.428
Neutrophil	1.036	0.338–3.173	0.950
Lymphocyte	0.648	0.236–1.776	0.399
CRP	2.620	1.037–6.594	0.041
PCT	1.838	0.589–5.734	0.294
BNP	3.080	0.776–12.221	0.110
FIB	2.781	0.828–9.347	0.098
D-D	0.548	0.137–2.194	0.395
APTT	0.421	0.124–1.431	0.166
PT	0.600	0.205–1.760	0.353

Abbreviations: BMI, body mass index; WBC, white blood cell count; CRP, C-reactive protein; PCT, procalcitonin; BNP, brain natriuretic peptide; FIB, fibrinogen; D-D, D-dimer; APTT, activated partial thromboplastin time; PT, prothrombin time.

Figure 3 Survival analysis. Kaplan–Meier curve of severe pneumonia group with different miR-10a-3p levels. Patients with low miR-10a-3p level had a higher survival rate. Log Rank P = 0.022.