CCL20/CCR6 Mediated Macrophage Activation and Polarization Can Promote Adenoid Epithelial Inflammation in Adenoid Hypertrophy

Figures & data

Table 1 Relevant Information of the Clinical Samples from AH Patients

Sample	Gender	Age (Years)	Duration of Lesion (in Months)	CCL20 (pg/mL)	CCR6 (pg/mL)
1	Male	5	24	195.7	55.1
2	Female	5	6	236.2	64
3	Male	6	36	245.6	67.6
4	Male	3	6	246.6	68.4
5	Male	6	12	247.7	72.1
6	Male	8	36	237.3	69.2
7	Female	3	6	252.9	73.4
8	Female	8	48	214.4	60.6
9	Female	7	24	259.1	71.5
10	Male	6	24	298.7	80.7
11	Female	5	12	248.7	73.4
12	Male	7	24	245.6	64.8
13	Male	7	36	240.4	67.6
14	Male	9	24	252.9	74.2
15	Female	5	12	269.5	75.5
16	Female	12	72	284.1	81.7
17	Male	11	48	274.7	71.3
18	Male	12	24	283.1	72.3
19	Male	11	48	273.7	79.9

Figure 1 (A) H&E staining was used to detect the pathological state of the adenoids of AH patients. (B) IHC based detection of the expressions of CD86, CD206, IL-17A and FOXP3.

Figure 2 (A and B) Detection of polarization of macrophages by IF.

Figure 3 (A) CCK-8 assay for identifying the proliferation of lymphocytes. (B) IF mediated detection of the expressions of CD4, IL-17 and FOXP3. (C) Detection of the levels of IL-17, IL-10 and TGF-β by ELISA. *P<0.05, **P<0.01, ***P<0.001 vs control; ^#P<0.05, ^###P<0.001 vs M0. All experiments were performed in triplicate and the data are presented as mean ± SD.

Figure 4 (A) CCK-8 assay for identifying the proliferation of adenoid epithelial cells. (B) Detection of the levels of IL6, TNF-α, IL-1β and CCL20 by ELISA. *P<0.05, **P<0.01 vs control; ^#P<0.05, ^##P<0.01, ^###P<0.001 vs M0; ^ΔP<0.05, ^ΔΔP<0.01, ^ΔΔΔP<0.001 vs M1. All experiments were performed in triplicate and the data are presented as mean ± SD.

Figure 5 (A) Detection of the expression of CCR6 by Western blot. (B) Detection of the expression of inflammation related proteins by Western blot. *P<0.05 vs control; ^#P<0.05, ^###P<0.001 vs M0; ^ΔP<0.05, ^ΔΔP<0.01, ^ΔΔΔP<0.001 vs M1. All experiments were performed in triplicate and the data are presented as mean ± SD.