NUP62CL as an Immunological and Prognostic Biomarker of Oral Squamous Cell Carcinoma

Figures & data

Figure 1 (A) NUP62CL mRNA expression in normal and OSCC tumor tissues. (B) NUP62CL mRNA expression in OSCC tumor tissues and their paired normal tissue. (C) Patients with high NUP62CL expression had a poor prognosis. ***P < 0.001.

Figure 2 The multiplex immunohistochemistry revealed the expression of the NUP62CL protein in tumor tissues (A) and normal tissues (B).

Table 1 Relationship Between NUP62CL Expression and Clinicopathological Features

Characteristic	Samples	NUP62CL	NUP62CL	Pearson	P
		Low Expression	High Expression	χ^2
Total	319	160 (50.16%)	159 (49.84%)
Sex
Male	143	78 (54.55%)	65 (45.45%)
Female	176	82 (46.59%)	94 (53.41%)	1.997	0.158
Age
>60	165	83 (50.30%)	82 (49.70%)
≤60	154	77 (50.00%)	77 (50.00%)	0.003	0.957
Anatomic classification
Buccal Mucosa	127	63 (49.61%)	64 (50.39%)
Oral Tongue	169	86 (50.89%)	83 (49.11%)	0.048	0.827
Other oral area	23	11	12
T stage
T1	187	110 (58.82%)	77 (41.18%)
T2	110	48 (43.64%)	62 (56.36%)	6.416	0.011*
T3 & T4	22	2	20
N stage
N0	273	135 (49.45%)	138 (50.55)
N1 & N2	46	25 (54.35%)	21 (45.65)	0.378	0.539
M stage
M0	319	160	159
Clinical stage
I	169	99 (58.58%)	70 (41.42%)
II	88	35 (39.77%)	53 (60.23%)
III & V	62	26 (41.94%)	36 (58.06%)	10.268	0.006**

Notes: *P < 0.05; **P < 0.01.

Abbreviations: T, tumor size; N, lymph node metastasis; M, distant metastasis.

Figure 3 Univariate and multivariable analyses of prognostic factors for 5-year survival in patients with OSCC. *P<0.05, **P<0.01, ***P<0.001.

Figure 4 The impacts of NUP62CL expression levels (A), age (B), anatomic classification (C), T stage (D), N stage (E) and clinical stage (F) on the overall survival of OSCC patients.

Figure 5 (A) The group comparison diagram represents the proportions of 24 distinct immune cell types. (B) NUP62CL expression was correlated with tumor-infiltrating immune cells (TIICs) abundance. *P<0.05, **P<0.01, ***P<0.001.

Figure 6 (A) Representative multiplex immunofluorescence staining images detecting different immune cell types in OSCC tissues. (B) The infiltration levels of TIICs in OSCC. (C) NUP62CL expression was related to TIICs abundance. *P<0.05, **P<0.01.

Figure 7 (A) The representative mIHC images of PD-1, PD-L1 and CTLA-4 protein expression. (B) The PD-1, PD-L1 and CTLA-4 protein expression in NUP62CL high-expression and low-expression groups. The correlation of NUP62CL protein expression with the immune checkpoints in OSCC tissues, including PD-1 (C), PD-L1 (D) and CTLA-4 (E). *P<0.05, ***P<0.001.