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ORIGINAL RESEARCH

Radix Salvia miltiorrhiza Ameliorates Burn Injuries by Reducing Inflammation and Promoting Wound Healing

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Pages 4251-4263 | Received 21 Jun 2023, Accepted 16 Sep 2023, Published online: 25 Sep 2023

Figures & data

Table 1 Gradient Elution Condition

Table 2 Effects on Apparent Scores of Rats in Burn Injury Model

Figure 1 Effect on the burn area of rats. (a) Pictures of rats with burns at different time periods. (bd) Burn area at different time periods. (e) Healing rate of burns (100%). **P < 0.01 vs BG group. ##P < 0.01 vs MG group.

Abbreviations: BG, blank group; MG, model group; JWHG, Jinwanhong group; RSMHG, RSM high-dose group; RSMLG, RSM low-dose group.
Figure 1 Effect on the burn area of rats. (a) Pictures of rats with burns at different time periods. (b–d) Burn area at different time periods. (e) Healing rate of burns (100%). **P < 0.01 vs BG group. ##P < 0.01 vs MG group.

Figure 2 Effects on blood perfusion of burn tissue. (a) Pictures of blood perfusion volume at different time periods. (bd) Burn area at different time periods. **P < 0.01 vs BG group. ##P < 0.01, #P < 0.05 vs MG group.

Abbreviations: BG, blank group; MG, model group; JWHG, Jinwanhong group; RSMHG, RSM high-dose group; RSMLG, RSM low-dose group.
Figure 2 Effects on blood perfusion of burn tissue. (a) Pictures of blood perfusion volume at different time periods. (b–d) Burn area at different time periods. **P < 0.01 vs BG group. ##P < 0.01, #P < 0.05 vs MG group.

Figure 3 Effect of serum biochemical indicators. (ac) Levels of inflammatory cytokines. (d) Levels of collagen.(ef) Levels of growth factors. **P < 0.01 vs BG group. ##P < 0.01 vs MG group.

Abbreviations: BG, blank group; MG, model group; JWHG, the Jinwanhong group; RSMHG, RSM high-dose group; RSMLG, RSM low-dose group.
Figure 3 Effect of serum biochemical indicators. (a–c) Levels of inflammatory cytokines. (d) Levels of collagen.(e–f) Levels of growth factors. **P < 0.01 vs BG group. ##P < 0.01 vs MG group.

Figure 4 Effect on histopathological changes. (a) Pathological photos of HE staining (×100). (b) Description of grading criteria. (c) Ridit test. **P < 0.01 vs BG group. ##P < 0.01 vs MG group.

Abbreviations: BG, blank group; MG, model group; JWHG, Jinwanhong group; RSMHG, RSM high-dose group; RSMLG, RSM low-dose group.
Figure 4 Effect on histopathological changes. (a) Pathological photos of HE staining (×100). (b) Description of grading criteria. (c) Ridit test. **P < 0.01 vs BG group. ##P < 0.01 vs MG group.

Figure 5 Effect on protein expression of growth factor. (a) Immunohistochemistry pictures (×400). (be) Protein expression of growth factor. **P < 0.01 vs BG group. ##P < 0.01, #P < 0.05 vs MG group.

Abbreviations: BG, blank group; MG, model group; JWHG, Jinwanhong group; RSMHG, RSM high-dose group; RSMLG, RSM low-dose group.
Figure 5 Effect on protein expression of growth factor. (a) Immunohistochemistry pictures (×400). (b–e) Protein expression of growth factor. **P < 0.01 vs BG group. ##P < 0.01, #P < 0.05 vs MG group.

Figure 6 Absorption of tanshinone IIA and salvianolic acid B in different tissues. (a) Molecular structure of tanshinone IIA. (b) Molecular structure of salvianolic acid B. (c) Absorption in different tissues of salvianolic acid B. (d) UHPLC -LTQ- Orbitrap- MS of salvianolic acid B in biological samples. (e) Absorption in different tissues of tanshinone IIA. (f) UHPLC-LTQ-Orbitrap-MS of tanshinone IIA in biological samples.

Abbreviations: BG, blank group; RSMHG, RSM high-dose group; RSMLG, RSM low-dose group.
Figure 6 Absorption of tanshinone IIA and salvianolic acid B in different tissues. (a) Molecular structure of tanshinone IIA. (b) Molecular structure of salvianolic acid B. (c) Absorption in different tissues of salvianolic acid B. (d) UHPLC -LTQ- Orbitrap- MS of salvianolic acid B in biological samples. (e) Absorption in different tissues of tanshinone IIA. (f) UHPLC-LTQ-Orbitrap-MS of tanshinone IIA in biological samples.