A Review: Matrix Metallopeptidase-9 Nanoparticles Targeted for the Treatment of Diabetic Foot Ulcers

Figures & data

Table 1 Summary of the Matrix Metalloproteinase-9 Treatment Articles That Were Previously Published and Included Studies in the Review, 2021

Treatment	Study Material	Method of MMP Measuring	Effect on MMPs	Reference
Vacuum-assisted closure (VAC)	Human chronic wound fluid	Gelatin zymography	Reduced MMP-9 and MMP-2	[^Citation78]
VAC	Human chronic wound fluid	ELISA	Reduced MMP-9/TIMP-1 ratio, no change in MMP-9	[^Citation79]
Mesenchymal stem cells (MSC)	Mouse model of diabetic wounds	Gelatin zymography, quantitative PCR	Reduced MMP-9 activity and expression	[^Citation80]
N-acetyl cysteine	HaCat cells treated with MGO	Western blot	Reduced MMP-9 expression	[^Citation81]
Manuka honey wound dressing	Human DFU patient wound fluid	ELISA	Increased MMP-9 expression (no improvement on ulcer healing)	[^Citation82]
Becaplermin	Mouse model of diabetic wounds	In-situ zymography and batimastat affinity resin coupled proteomics	Decreased gelatinase activity, no effect on collagenase activity; decreased active MMP-9, no effect on active MMP-8	[^Citation83]
Aclerastide	Mouse model of diabetic wounds	Batimastat affinity resin coupled proteomics	Increased active MMP-9, no effect on active MMP-8	[^Citation84]

Abbreviations: ELISA, enzyme-linked immunosorbent assay; DFU, diabetic foot ulcer; MMP-8, matrix metalloproteinase-8; MMP-9, matrix metalloproteinase-9; PCR, polymerase chain reaction; VAC, vacuum-assisted closure; MGO, methylglyoxal; HaCaT, cultured human keratinocyte (cells); THMM-1, tissue inhibitor matrix metalloproteinase-1.

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Nguyen TT, Ding D, Wolter WR, et al. Expression of active matrix metalloproteinase-9 as a likely contributor to the clinical failure of aclerastide in treatment of diabetic foot ulcers. Eur J Pharmacol. 2018;834:77–83. doi:10.1016/j.ejphar.2018.07.014

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