https://orcid.org/0000-0001-8083-5873
Figures & data
Figure 1 Expression level of microRNA-1 (miR-1) 48h and 96h in U87 cells after transfection with scrambled microRNA-mimic (Scr) or microRNA-1 mimics (miR-1). Absolute qPCR results (raw Cq values); n=3, *p < 0.05.
Figure 2 Protein expression level of Connexin 43 (Cx43) in U87 cells 48 h and 96h after transfection with scrambled microRNA-mimic (Scr) or microRNA-1 mimic (miR-1). Determined by Western blot analysis; n=3, *p < 0.05.
Figure 3 (A) Relative microRNA-1 (miR-1) expression in sciatic nerve (n=4) and dorsal root ganglia (DRG; n=5) of CCI rats that received intraneural miR-1-mimic injections on day 0, 3, and 7 vs rats that received scrambled-microRNA mimics (Scr). (B) Operation site during intraneural injections of miR-1. As depicted, injections were placed proximally to the ligatures; *p < 0.05 vs Scrambled.
Figure 4 (A) Protein levels of Connexin 43 (Cx43) in sciatic nerves after microRNA-1 (miR-1) or scrambled (Scr) miRNA-injection. No significant changes were found in CX43 protein levels in sciatic nerves treated with miR-1 mimetic nucleotides in comparison to nerves treated with scrambled miRNAs. (B) Paw withdrawal thresholds of rats after chronic constriction injury (CCI) of the sciatic nerve and intraneural injection with scrambled microRNA mimic (Scr) or microRNA-1 mimic (miR-1). Mimics were injected at day 0, 3, 7 after CCI. Thresholds were detected at day 10 after CCI (n=16–21, n.s.).
