Impact of smoking on frequency and spectrum of K-RAS and EGFR mutations in treatment naive Indonesian lung cancer patients

Figures & data

Table 1 Demography

Characteristics	N (143)	Percent
Gender
Male	102	71%
Female	41	29%
Age
Median	55
Average	53.7
Range	26‒84
Smoking History
Ever smokers (ES)	89	62%
Never smokers (NS)	51	36%
Unknown	3
All adenocarcinoma
EGFR Genotypes
Wild type (normal)	52	36%
Mutations	91	64%
Common Mutations (Exon 19 Dels, L858R)	61	67%
Uncommon mutations (G719X, L861Q, T790M)	17	19%
Mix mutations	13	14%
K-RAS Genotypes
Wild type	133	93%
Mutations	10	7.0%
Transversion	6	60%
G12C	4
G12A	1
G12R	1
Transition	4	40%
G12D	3
G12S	1
Mix EGFR and K-RAS concomitant mutations	4	2.8%
K-RAS mutations in 91 EGFR Mutant patients		4.4%
EGFR mutations in 10 K-RAS mutant patients		40%

Figure 1 EGFR and K-RAS mutation rates in males and females with or without smoking history.

Abbreviations: ES, ever smokers; NS, nonsmokers.

Table 2 Impact of smoking history to prevalence and spectrum of EGFR mutation

Characteristics			EGFR genotypes				*p-value	EGFR mutation types						*p-value
			Wild types		Mutants			Common		Uncommon		Mix
Smoking history
	Ever smokers	89	38	43%	51	57%	0.0465	33	65%	8	16%	10	20%	0.0632
	Never smokers	51	13	25%	38	75%		27	71%	9	24%	2	5%
	Unknown	3
Age 55 years old and younger
	Ever smokers	51	24	47%	27	53%	0.0343	15	56%	7	26%	5	19%	0.139
	Never smokers	21	4	19%	17	81%		11	65%	6	35%	0	0%
Age older than 55 years old
	Ever smokers	38	14	37%	24	63%	0.6127	18	75%	1	4%	5	21%	0.421
	Never smokers	30	9	30%	21	70%		16	76%	3	14%	2	10%
Male
	Ever smokers	84	36	43%	48	57%	0.06	30	63%	8	17%	10	21%	0.429
	Never smokers	17	3	18%	14	82%		10	71%	3	21%	1	7%
Female
	Ever smokers	5	2	40%	3	60%	0.63	3	100%	0	0%	0	0%	1
	Never smokers	34	10	29%	24	71%		17	71%	6	25%	1	4%

Notes: * p-value <0.05 were statistically significant.

Table 3 Impact of smoking history to prevalence and spectrum of K-RAS mutation

			K-RAS WT		K-RAS mutation		*p-value	Transversion		Transitions
		Total	n	Rate	n	Rate		n	Rate	n	Rate
		140	131	94%	9	6%
Smoking history
	Ever smokers	89	83	93%	6	7%	1.00	4	67%	2	33%
	Never smokers	51	48	94%	3	6%		1	33%	2	67%
	Unknown	3
55 years and under
	Ever smokers	51	47	92%	4	8%	1.00	2	50%	2	50%
	Never smokers	21	19	90%	2	10%		0	0%	2	100%
Older than 55 years
	Ever smokers	38	36	95%	2	5%	1.00	2	100%	0	0%
	Never smokers	30	29	97%	1	3%		1	100%	0	0%
Male		101	95	94%	6	6%
	Ever smokers	84	78	93%	6	7%	0.56	4	67%	2	33%
	No smokers	17	17	100%	0	0%		0	-	0	-
Female		39	36	93%	3	7%
	Ever smokers	5	5	100%	0	0%	1.00	0	-	0	-
	Never smokers	34	31	91%	3	9%		1	33%	2	67%

Table 4 Clinical characteristic of EGFR and K-RAS double mutations

Gender	Age (years)	Smoking history	EGFR in cytology	K-RAS mutation	K-RAS mutation types
Female	84	Never smoker	L858R	G12C	Transversion
Male	46	Unknown	T790M, G719S, L858R	G12C	Transversion
Male	42	Ever smoker	G719S	G12S	Transition
Female	41	Never smoker	L861Q, T790M	G12D	Transition