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Original Research

Utility Of An Automatic Limulus Amebocyte Lysate Kinetic Turbidimetric Test For Endotoxin Screening Of Dialysate Samples

, , , , , , , , ORCID Icon & show all
Pages 429-433 | Published online: 07 Oct 2019

Figures & data

Figure 1 (A) Automatic LAL test device (Toxinometer ET-mini™). The device measures 27.7 cm (length)×17.6 cm (width)×8.5 cm (height), with a total weight of 2.4 kg. (B) The Toxinometer ET-mini can simultaneously measure four samples.

Abbreviation: LAL, limulus amebocyte lysate.
Figure 1 (A) Automatic LAL test device (Toxinometer ET-mini™). The device measures 27.7 cm (length)×17.6 cm (width)×8.5 cm (height), with a total weight of 2.4 kg. (B) The Toxinometer ET-mini can simultaneously measure four samples.

Figure 2 Principles of endotoxin activity measurement using the automatic LAL test (Toxinometer ET-mini™). (A) Endotoxin activity is determined from the time required for the transmitted light ratio to decrease below a certain threshold (92% of the initial value), which is defined as the gelation time. (B) The log value of endotoxin activity was calculated from the log–log plot of gelation time.

Abbreviation: LAL, limulus amebocyte lysate.
Figure 2 Principles of endotoxin activity measurement using the automatic LAL test (Toxinometer ET-mini™). (A) Endotoxin activity is determined from the time required for the transmitted light ratio to decrease below a certain threshold (92% of the initial value), which is defined as the gelation time. (B) The log value of endotoxin activity was calculated from the log–log plot of gelation time.

Figure 3 Correlation between endotoxin activity levels measured using the automatic LAL test and the chromogenic substrate LAL test.

Abbreviation: LAL, limulus amebocyte lysate.
Figure 3 Correlation between endotoxin activity levels measured using the automatic LAL test and the chromogenic substrate LAL test.

Figure 4 Analysis of systematic errors between the automatic LAL test and the chromogenic substrate LAL test.

Abbreviation: LAL, limulus amebocyte lysate.
Figure 4 Analysis of systematic errors between the automatic LAL test and the chromogenic substrate LAL test.