Altered intrinsic brain activities in patients with acute eye pain using amplitude of low-frequency fluctuation: a resting-state fMRI study

Figures & data

Table 1 Demographics and clinical measurements by group

Condition	EP	HC	t	p-value*
Male/female	15/5	15/5	N/A	>0.99
Age (years)	37.85±5.63	38.00±5.62	−0.084	0.933
Weight (kg)	69.30±6.04	69.95±5.89	−0.344	0.732
Handedness	20R	20R	N/A	>0.99
Duration of EP (days)	6.80±2.12	N/A	N/A	N/A

Notes:

* p<0.05. Independent t-tests comparing two groups.

Abbreviations: EP, eye pain; HC, healthy control; N/A, not applicable.

Figure 1 Spontaneous brain activity in the EPs versus HCs.

Notes: (A and B) Significant activity differences were observed in the LPreCG/PosCG, RPreCG/PosCG, LPrecu, RParaHG, LParaHG, and left caudate. The red or yellow denotes higher ALFF values, and the blue areas indicate lower ALFF values, respectively (p<0.01 for multiple comparisons using Gaussian-random field theory, z>2.3, p<0.01, cluster >40 voxels, FDR corrected). (C) The mean values of altered ALFF values between the EPs and HC groups.
Abbreviations: ALFF, amplitude of low-frequency fluctuation; EP, eye pain; FDR, false discovery rate; HC, healthy control; LParaHG, left parahippocampal gyrus; LPreCG/PosCG, left precentral/postcentral gyrus; LPrecu, left precuneus; RParaHG, right parahippocampal gyrus; RPreCG/PosCG, right precentral/postcentral gyrus.

Table 2 Brain areas with significantly different ALFF values between groups

Brain regions/conditions	BA	MNI coordinates			Cluster size	t-value
		X	Y	Z
EP < HCs
Left precentral/postcentral gyrus	3/4	−48	−18	48	53	−3.593
Right precentral/postcentral gyrus	3/4	51	−12	48	80	−4.074
Left precuneus	7	3	−51	57	54	−4.374
EP > HCs
Right parahippocampal gyrus	36	39	−21	−21	41	5.002
Left parahippocampal gyrus	36	−36	−36	−12	63	5.803
Left caudate	–	−12	18	−3	112	5.154

Note: The statistical threshold was set at the voxel level with p<0.05 for multiple comparisons using Gaussian random-field theory (z>2.3, p<0.01, cluster >40 voxels, FDR corrected).

Abbreviations: ALFF, amplitude of low-frequency fluctuation; BA, Brodmann area; EP, eye pain; FDR, false discovery rate; HC, healthy control; L, left; MNI, Montreal Neurological Institute; R, right.

Figure 2 ROC curve analysis of the mean ALFF values for altered brain regions.

Notes: (A) The areas under the ROC curve were 0.827 (p<0.001; 95% CI: 0.696–0.959) for LPreCG/PosCG; RPreCG/PosCG (0.873) (p<0.001; 95% CI: 0.766–0.979); LPrecu (0.847) (p<0.001; 95% CI: 0.725–0.970), (EPs < HCs). (B) RParaHG (0.905) (p<0.001; 95% CI: 0.815–0.995) and LParaHG (0.910) (p<0.001; 95% CI: 0.820–1.000); left caudate (0.947) (p<0.001; 95% CI: 0.884–1.000) (EPs > HCs).
Abbreviations: ALFF, amplitude of low-frequency fluctuation; CI, confidence interval; EP, eye pain; HC, healthy control; LParaHG, left parahippocampal gyrus; LPreCG/PosCG, left precentral/postcentral gyrus; LPrecu, left precuneus; RParaHG, right parahippocampal gyrus; RPreCG/PosCG, right precentral/postcentral gyrus; ROC, receiver operating characteristic.