Platelet membrane receptor P2Y12 H1/H2 polymorphism is highly associated with cerebral infarction: a case–control study

Figures & data

Table 1 Comparison of demographic characteristics and risk factors of stroke between the two groups

Category	CI group (n=152)	Contrast group (n=168)	P-value
Age, mean±SD	63.91±11.486	61.65±11.486	0.084
Gender (M/F), n	83/69	78/90	0.148
Smoking, n (%)	19.1 (29)	12.5 (21)	0.124
Alcohol, n (%)	16.4 (25)	10.7 (18)	0.143
SBP (mmHg), mean±SD	156.82±24.015	140.04±24.438	<0.001
DBP (mmHg), mean±SD	90.15±14.543	82.58±12.593	<0.001
Blood glucose (mmol/L), mean±SD	6.1438±2.114	5.757±1.46	0.06
Cholesterol (mmol/L), mean±SD	4.5943±1.192	4.836±1.1206	0.063
Triglyceride (mmol/L), mean±SD	1.553±0.9221	1.719±1.1419	0.156
Low-density lipoprotein (mmol/L), mean±SD	2.9268±0.9681	3.006±0.9356	0.46
High-density lipoprotein (mmol/L), mean±SD	1.104±0.3444	1.1407±0.339	0.334
Fibrinogen (g/L), mean±SD	3.339±0.8741	3.091±0.6764	0.005
Serum uric acid (µmol/L), mean±SD	271.59±80.5959	278.26±94.5859	0.5
Homocysteine levels (µmol/L), mean±SD	16.628±12.0426	14.78±9.494	0.127

Abbreviations: CI, cerebral infarction; M, male; F, female; SBP, systolic blood pressure; DBP, diastolic blood pressure.

Figure 1 P2Y12 gene electrophoretogram.

Notes: M denotes the indicator “Mark”: the index strip from top to bottom is 250 bp, 220 bp, 200 bp, 170 bp and 150 bp. (1 and 6) The PCR product: 230 bp. (2) H1/H1: 197 bp; (3 and 5) H1/H2: 197 bp and 157 bp. (4) H2/H2: 157 bp. Magnification 1:1.

Table 2 Frequency comparison of the H1/H2 polymorphism of the P2Y12 gene between CI and contrast groups

Group	n	Genotype			χ^2	P-value	Allele type		χ^2	P-value
		H1/H1 (%)	H1/H2 (%)	H2/H2 (%)			H1 (%)	H2 (%)
CI group	152	112 (73.7)	36 (23.4)	4 (2.9)	4.440	0.035	260 (85.5)	44 (14.5)	5.392	0.020
Contrast group	168	140 (83.3)	27 (16.1)	1 (0.6)			307(91.4)	29 (8.6)

Abbreviation: CI, cerebral infarction.

Table 3 Multivariate logistic regression analysis with CI as the dependent variable

Independent variable	Significance	Exp (B)	95% confidence interval of Exp (B)
			Lower limit	Upper limit
Age	0.596	1.007	0.982	1.031
Gender	0.334	0.747	0.414	1.349
Smoking	0.585	1.257	0.553	2.855
Drinking	0.409	1.451	0.600	3.505
SBP	0.004	1.002	1.007	1.037
DBP	0.150	1.019	0.993	1.045
Blood glucose	0.150	1.140	0.954	1.362
Cholesterol	0.166	0.761	0.517	1.120
Triglyceride	0.440	0.887	0.653	1.203
Low-density lipoprotein	0.741	1.078	0.691	1.681
High-density lipoprotein	0.508	0.748	0.317	1.766
Plasma fibrinogen level	0.047	1.413	1.005	1.986
Serum uric acid	0.195	0.998	0.995	1.001
Homocysteine level	0.217	1.015	0.991	1.039
Genotype	0.038	1.928	1.137	3.188

Abbreviations: CI, cerebral infarction; SBP, systolic blood pressure; DBP, diastolic blood pressure.

Table 4 The genotype distribution of the Hardy–Weinberg equilibrium in the two groups

	P2y12 g52t
	GG	GT	TT
CI group
Observed value	112	36	4
Expected value	111.1	37.69	3.2
	χ^2=0.289, P>0.75
Contrast group
Observed value	140	27	1
Expected value	140.28	26.4	1.18
	χ^2=0.0426, P>0.75

Abbreviation: CI, cerebral infarction.