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Neuropsychiatric Disease and Treatment Volume 15, 2019 - Issue
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Original Research

Antidepressant, anti-inflammatory, and antioxidant effects of electroacupuncture through sonic hedgehog–signaling pathway in a rat model of poststroke depression

Wa Cai1 Institute of Acupuncture and Anesthesia, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China;2 Department of Acupuncture, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China
,
Wen Ma1 Institute of Acupuncture and Anesthesia, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China
,
Guan-Tao Wang1 Institute of Acupuncture and Anesthesia, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China
,
Yi-Jing Li1 Institute of Acupuncture and Anesthesia, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China
&
Wei-Dong Shen1 Institute of Acupuncture and Anesthesia, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China;2 Department of Acupuncture, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of ChinaCorrespondence[email protected]
Pages 1403-1411 | Published online: 23 May 2019

Figures & data

Figure 1 Protocol for the experiment. All other experimental procedures were done after the interventions. Rats were killed after the last test.

Abbreviations: SPT, sucrose-preference test; LA, locomotor activity; SAC, sacrifice; MCAO, middle cerebral artery occlusion; WB, Western blot.
Figure 1 Protocol for the experiment. All other experimental procedures were done after the interventions. Rats were killed after the last test.

Figure 2 Improvement in depressive-like behaviors of PSD. (A) Effect of EA treatment on SPT; (B) changes in locomotion activity after EA treatment. Values are presented as means ± SEM, with eight rats in each group. **P<0.01 versus sham group; ***P<0.001 versus sham group; ##P<0.01 versus PSD group; ###P<0.001 versus PSD group; &&P<0.01 versus EA group; %P<0.05 versus EA + Cyc group.

Abbreviations: PSD, poststroke depression; EA, electroacupuncture; SPT, sucrose-preference test; Cyc, cyclopamine.
Figure 2 Improvement in depressive-like behaviors of PSD. (A) Effect of EA treatment on SPT; (B) changes in locomotion activity after EA treatment. Values are presented as means ± SEM, with eight rats in each group. **P<0.01 versus sham group; ***P<0.001 versus sham group; ##P<0.01 versus PSD group; ###P<0.001 versus PSD group; &&P<0.01 versus EA group; %P<0.05 versus EA + Cyc group.

Figure 3 Alleviation of oxidative stress and inflammation. (A–F) Comparisons of SOD, GSH, MDA, IL6, TNFα, and IL1β levels in sham, PSD, EA + PSD, EA + Cyc, EA + vehicle, and Flu groups by ELISA. Values are presented as means ± SEM, with eight rats in each group. **P<0.01 versus sham group; #P<0.05 versus PSD group; ###P<0.001 versus PSD group.

Abbreviations: MDA, malondialdehyde; PSD, poststroke depression; EA, electroacupuncture; Cyc, cyclopamine; Flu, fluoxetine.
Figure 3 Alleviation of oxidative stress and inflammation. (A–F) Comparisons of SOD, GSH, MDA, IL6, TNFα, and IL1β levels in sham, PSD, EA + PSD, EA + Cyc, EA + vehicle, and Flu groups by ELISA. Values are presented as means ± SEM, with eight rats in each group. **P<0.01 versus sham group; #P<0.05 versus PSD group; ###P<0.001 versus PSD group.

Figure 4 Changes in 5HT levels. Comparison of 5HT levels in sham, PSD, EA + PSD, EA + Cyc, EA + vehicle, and Flu groups by ELISA. Values are presented as means ± SEM, with eight rats in each group. **P<0.01 versus sham group; ##P<0.05 versus PSD group; &P<0.05 versus EA group; %P<0.05 versus EA + Cyc group.

Abbreviations: PSD, poststroke depression; EA, electroacupuncture; Cyc, cyclopamine; Flu, fluoxetine.
Figure 4 Changes in 5HT levels. Comparison of 5HT levels in sham, PSD, EA + PSD, EA + Cyc, EA + vehicle, and Flu groups by ELISA. Values are presented as means ± SEM, with eight rats in each group. **P<0.01 versus sham group; ##P<0.05 versus PSD group; &P<0.05 versus EA group; %P<0.05 versus EA + Cyc group.

Figure 5 Regulation of Shh-signaling pathway. (A) Immunoblot analysis for expression of Shh, Gli1, Smo, Ptch1, and β-actin in sham, PSD, EA + PSD, EA + Cyc, EA + vehicle, and Flu groups. (B–E) Comparisons of Shh, Gli1, Smo, and Ptch1 levels in six groups by Western blot. Values are presented as means ± SEM, with eight rats in each group. *P<0.05 versus PSD group; **P<0.01 versus PSD group; ***P<0.001 versus PSD group; #P<0.05 versus PSD group; ##P<0.01 versus PSD group; &P<0.05 versus EA group; %P<0.05 versus the EA + Cyc group.

Abbreviations: PSD, poststroke depression; EA, electroacupuncture; Cyc, cyclopamine; Flu, fluoxetine.
Figure 5 Regulation of Shh-signaling pathway. (A) Immunoblot analysis for expression of Shh, Gli1, Smo, Ptch1, and β-actin in sham, PSD, EA + PSD, EA + Cyc, EA + vehicle, and Flu groups. (B–E) Comparisons of Shh, Gli1, Smo, and Ptch1 levels in six groups by Western blot. Values are presented as means ± SEM, with eight rats in each group. *P<0.05 versus PSD group; **P<0.01 versus PSD group; ***P<0.001 versus PSD group; #P<0.05 versus PSD group; ##P<0.01 versus PSD group; &P<0.05 versus EA group; %P<0.05 versus the EA + Cyc group.

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