The neuroprotective mechanism of 2-arachidonoylglycerol 2-AG against non-caspase-dependent apoptosis in mice hippocampal neurons following MCAO

Figures & data

Table 1 Effects of 2-AG on apoptosis rate of hippocampal neurons, the expression of AIF, Endo G and BNIP3 in mice (± s)

Group	Number	Apoptosis rate (%)	Red/Green	AIF mRNA	Endo G mRNA	BNIP3 mRNA	AIF protein	Endo G protein	BNIP3 protein
Sham group	10	14.35±2.04	7.54±0.64	1	1	1	1.57±0.18	1.28±0.25	1.32±0.34
Model group	10	27.67±4.32**	1.64±0.12**	2.68±0.52**	2.82±0.57**	2.42±0.41**	0.32±0.27**	0.41±0.43**	0.45±0.49**
2-AG group	10	18.95±2.17^##	6.15±0.47^##	1.55±0.12^##	1.37±0.21^##	1.27±0.19^##	1.18±0.15^##	1.17±0.29^##	1.21±0.31^##

Notes: Compared with Sham group, **P<0.01; compared with Model group, ^##P<0.01.

Table 2 Effects of endogenous cannabinoids 2-AG on mitochondrial membrane potential of hippocampal neurons in mice (± s)

Group	Number	Red/Green
Sham group	10	7.54±0.64
Model group	10	1.64±0.12**
2-AG group	10	6.15±0.47^##

Notes: Compared with Sham group, **P<0.01; compared with Model group, ^##P<0.01.

Table 3 Effects of endogenous cannabinoids 2-AG on the expression of AIF mRNA in hippocampal tissues of mice in each group (± s)

Group	Number	Relative mRNA level of AIF
Sham group	10	1
Model group	10	2.68±0.52**
2-AG group	10	1.55±0.12^##

Notes: Compared with Sham group, **P<0.01; compared with Model group, ^##P<0.01.

Table 4 Effects of endogenous cannabinoids 2-AG on the expression of Endo G mRNA in hippocampal tissues of mice in each group (± s)

Group	Number	Relative mRNA level of Endo G
Sham group	10	1
Model group	10	2.82±0.57**
2-AG group	10	1.37±0.21^##

Notes: Compared with Sham group, **P<0.01; compared with Model group, ^##P<0.01.

Table 5 Effects of endogenous cannabinoids 2-AG on the expression of BNIP3 mRNA in hippocampal tissues of mice in each group (± s)

Group	Number	Relative mRNA level of BNIP3
Sham group	10	1
Model group	10	2.42±0.41**
2-AG group	10	1.27±0.19^##

Notes: Compared with Sham group, **P<0.01; compared with Model group, ^##P<0.01.

Figure 1 Expression of mitochondrial AIF protein in hippocampal tissue of mice in each group.

Figure 2 Expression of mitochondrial Endo G protein in hippocampal tissue of mice in each group.

Figure 3 Expression of mitochondrial BNIP3 protein in hippocampal tissue of mice in each group.

Availability of data and material

The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.