Figures & data
Figure 1 sEH inhibitor improved recovery of neurological function at 1 and 3 days after MCAO.
Abbreviations: IS, ischemia; AUDA, 12-(3-adamantan-1-yl-ureido)-dodecanoic acid; MCAO, middle cerebral artery occlusion; sEH, soluble epoxide hydrolase; SEM, standard error of the mean.
![Figure 1 sEH inhibitor improved recovery of neurological function at 1 and 3 days after MCAO.](/cms/asset/ecfc6d6a-652c-484a-aaa5-a415b860e44a/dndt_a_210403_f0001_b.jpg)
Figure 2 sEH inhibition decreased the infarct volume after MCAO.
Abbreviations: AUDA, 12-(3-adamantan-1-yl-ureido)-dodecanoic acid; MCAO, middle cerebral artery occlusion; sEH, soluble epoxide hydrolase; SEM, standard error of the mean; TTC, triphenyltetrazolium chloride.
![Figure 2 sEH inhibition decreased the infarct volume after MCAO.](/cms/asset/46b4c51d-e157-4ce5-aa2d-a2ac7cefe67b/dndt_a_210403_f0002_c.jpg)
Figure 3 sEH inhibition modulated microglia/macrophages polarization after MCAO.
Abbreviations: AUDA, 12-(3-adamantan-1-yl-ureido)-dodecanoic acid; Iba-1, ionized calcium binding adaptor molecule 1; MCAO, middle cerebral artery occlusion; sEH, soluble epoxide hydrolase; SEM, standard error of the mean.
![Figure 3 sEH inhibition modulated microglia/macrophages polarization after MCAO.](/cms/asset/a5836b27-1632-4592-b9e4-8936ce872ed0/dndt_a_210403_f0003_c.jpg)
Figure 4 Flow cytometry analysis revealed that the sEH inhibitor suppresses M1 microglia and promotes M2 microglia polarization after MCAO.
Abbreviations: APC, allophycocyanin; AUDA, 12-(3-adamantan-1-yl-ureido)-dodecanoic acid; FITC, fluorescein isothiocyanate; MCAO, middle cerebral artery occlusion; PE, phycoerythrin; sEH, soluble epoxide hydrolase; SEM, standard error of the mean.
![Figure 4 Flow cytometry analysis revealed that the sEH inhibitor suppresses M1 microglia and promotes M2 microglia polarization after MCAO.](/cms/asset/14484f9a-8c2f-4503-b77d-b45fd8524f7c/dndt_a_210403_f0004_c.jpg)
Figure 5 Inhibition of sEH regulated mRNA expression of pro- and anti-inflammatory cytokines and antioxidant mediators at 1 day after MCAO.
Abbreviations: AUDA, 12-(3-adamantan-1-yl-ureido)-dodecanoic acid; CAT, catalase; HO-1, heme oxygenase-1; IL, interleukin; iNOS, inducible nitric oxidase synthase; MCAO, middle cerebral artery occlusion; MCP-1, monocyte chemoattractant protein-1; MMP-9, matrix metalloproteinase-9; Nrf2, nuclear factor erythroid 2-related factor 2; PPARγ, peroxisome proliferator-activated receptor-γ; sEH, soluble epoxide hydrolase; SEM, standard error of the mean; SOD-1, superoxide dismutase-1.
![Figure 5 Inhibition of sEH regulated mRNA expression of pro- and anti-inflammatory cytokines and antioxidant mediators at 1 day after MCAO.](/cms/asset/880bf100-582b-4a59-91a6-a0e9dac02380/dndt_a_210403_f0005_b.jpg)
Figure 6 sEH inhibition modulated HO-1 and NeuN expression in neuronal cells at 1 day after MCAO.
Abbreviations: AUDA, 12-(3-adamantan-1-yl-ureido)-dodecanoic acid; HO-1, heme oxygenase-1; Iba-1, ionized calcium binding adaptor molecule 1; MCAO, middle cerebral artery occlusion; NeuN, neuronal nuclei; sEH, soluble epoxide hydrolase; SEM, standard error of the mean; SOD-1, superoxide dismutase-1.
![Figure 6 sEH inhibition modulated HO-1 and NeuN expression in neuronal cells at 1 day after MCAO.](/cms/asset/044ed939-6375-4bfa-905c-e413040bcf12/dndt_a_210403_f0006_c.jpg)
Table S1 The detected target gene primers