Traditional Chinese Medicine Shenmayizhi Decoction Ameliorates Memory And Cognitive Impairment Induced By Scopolamine Via Preventing Hippocampal Cholinergic Dysfunction In Rats

Figures & data

Figure 1 The chromatographic profile of SMYZ decoction.

Notes: (A) Major compounds in SMYZ decoction compared with reference standards; a: gastrodin; b: ferulic acid; c: ginsenosides Rg1; d: ginsenosides Rb1; e: quercetin. (B) Details of retention time from 24 to 31 mins.
Abbreviation: SMYZ, Shenmayizhi decoction.

Figure 2 SMYZ treatment on learning and memory deficits induced by scopolamine.

Notes: (A) The effects of SMYZ on the escape latency in the MWM test after 4-week treatment in rats, including (A) P < 0.01, control group versus model group; (B) P < 0.01, SMYZ-H group versus model group. (B) The number of crossing over the platform position during the spatial probe test in the MWM test on the fifth day, * P < 0.05, versus the model group. (C) The percent of swimming distance in target quadrant during the spatial probe test in the MWM test on the fifth day, * P < 0.05, versus the model group. (D) The percent of swimming time in target quadrant during the spatial probe test in the MWM test on the fifth day, * P < 0.05, versus model group. Data are expressed as mean ± SEM (n=10).
Abbreviations: SMYZ, Shenmayizhi decoction; SMYZ-L, low-concentration SMYZ group; SMYZ-M, medium-concentration SMYZ group; SMYZ-H, high-concentration SMYZ group.

Figure 3 AChE, ChAT and M1 levels in the hippocampus of scopolamine-induced amnesia rats.

Notes: (A) AChE expression in the hippocampus, * P < 0.05, versus the model group. (B) ChAT expression in the hippocampus, * P < 0.05, versus the model group. (C) M1 expression in the hippocampus, * P < 0.05 and ** P < 0.01, versus the model group. Data are expressed as mean ± SEM (n=3).
Abbreviations: AChE, acetylcholinesterase; ChAT, cholineacetyltransferase; M1, acetylcholine M1 receptor.

Figure 4 Effect of SMYZ on the activities of AChE and ChAT in the hippocampus of scopolamine-treated rats.

Notes: (A) Activity of choline acetytransferase (AChE). (B) Activity of acetylcholine esterase (ChAT). Data are expressed as mean±SEM (n=5). * P < 0.05, ** P < 0.01 versus model group.