Association study of genetic variants of the ANGPTL3 gene and susceptibility to ischemic stroke

Figures & data

Table 1 Distributions of selected variables in ischemic stroke cases and healthy controls

	Cases (n=989)	Controls (n=990)	P-value
Age, years
<60	446 (45.1%)	451 (45.6%)	0.837
≥60	543 (54.9%)	539 (54.4%)
Sex
Male	623 (63.0%)	615 (62.1%)	0.689
Female	366 (37.0%)	375 (37.9%)
Smoking status
Yes	257 (26.0%)	269 (27.2%)	0.550
No	732 (74.0%)	721 (72.8%)
Drinking status
Yes	316 (31.9%)	301 (30.4%)	0.554
No	683 (68.1%)	689 (69.6%)
Body-mass index (kg/m^2)	25.1±6.7	24.8±6.1	0.311
Hypertension
Yes	592 (59.9%)	399 (40.3%)	<0.001
No	397 (40.1%)	591 (59.5%)
Diabetes
Yes	318 (32.2%)	201 (20.3%)	<0.001
No	671 (67.8%)	789 (79.7%)
Hypercholesterolemia
Yes	445 (45.0%)	328 (33.1%)	<0.001
No	544 (55.0%)	662 (66.9%)

Note: Bold values statistically significant.

Table 2 Genetic variants of the ANGPTL3 gene and susceptibility to ischemic stroke (IS)

SNP	IS Cases	Controls	Adjusted OR (95% CI)*	P-value
rs12048208
GG	706	720	1.00 (reference)
AG	257	250	1.09 (0.77–1.55)	0.632
AA	26	20	1.38 (0.73–2.62)	0.326
A vs G			1.12 (0.87–1.44)	0.369
rs6690733
AA	588	655	1.00 (reference)
AC	362	310	1.35 (1.10–1.66)	0.004
CC	39	25	1.81 (1.07–3.04)	0.026
C vs A			1.34 (1.13–1.59)	0.001
rs12563308
TT	851	815	1.00 (reference)
TC	132	161	0.82 (0.67–1.00)	0.046
CC	6	14	0.43 (0.18–1.00)	0.049
C vs T			0.77 (0.64–0.93)	0.007
rs72641123
CC	836	837	1.00 (reference)
CA	139	144	1.01 (0.97–1.04)	0.781
AA	14	9	1.62 (0.68–3.86)	0.276
A vs C			1.08 (0.68–1.71)	0.755

Note: *Adjusted for age, sex, smoking and drinking status, body-mass index, hypertension, diabetes, and hypercholesterolemia. Bold values statistically significant.

Table 3 Associations of ANGPTL3 variants and serum-lipid levels in controls

SNP	Controls	TG (mmol/L)	TC (mmol/L)	HDL-C (mmol/L)	LDL-C (mmol/L)
rs6690733
AA	655	1.32±0.62	4.75±1.01	1.91±0.55	2.71±0.82
AC + CC	335	1.38±0.57	4.96±0.94	1.93±0.59	2.85±0.85
P-value		0.139	0.002	0.597	0.012
rs12563308
TT	815	1.35±0.61	4.86±0.99	1.92±0.57	2.78±0.86
TC + CC	175	1.30±0.57	4.64±0.97	1.90±0.53	2.63±0.71
P-value		0.320	0.007	0.670	0.031

Note: Bold values statistically significant.

Figure 1 Effects of the promoter polymorphism rs6690733 of ANGPTL3 gene on transcription activity.

Notes: Transcription activity was measured using an in vitro luciferase assay, and results are shown as means ± SD. Data expressed as fold increase in luciferase activity relative to pGL3-A. One-way ANOVA was used to assess statistical significance.